Patents by Inventor Barbara J. Wold
Barbara J. Wold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10533177Abstract: Long noncoding RNAs (lncRNAs) are identified that enhance pluripotency reprogramming of somatic cells as well as differentiation of pluripotent cells. Induced pluripotent stem (iPS) cell generation from somatic cells leads to the upregulation and downregulation of identified lncRNAs. The modulation of these lncRNAs are capable of enhancing pluripotency of somatic cells as well as enhancing differentiation of a pluripotent cell.Type: GrantFiled: November 27, 2017Date of Patent: January 14, 2020Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Daniel H. Kim, Barbara J. Wold
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Patent number: 9862943Abstract: Long noncoding RNAs (lncRNAs) are identified that enhance pluripotency reprogramming of somatic cells as well as differentiation of pluripotent cells. Induced pluripotent stem (iPS) cell generation from somatic cells leads to the upregulation and downregulation of identified lncRNAs. The modulation of these lncRNAs are capable of enhancing pluripotency of somatic cells as well as enhancing differentiation of a pluripotent cell.Type: GrantFiled: May 4, 2015Date of Patent: January 9, 2018Assignee: California Institute of TechnologyInventors: Daniel H. Kim, Barbara J. Wold
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Patent number: 9441224Abstract: Long noncoding RNAs (lncRNAs) are identified that enhance pluripotency reprogramming of somatic cells as well as differentiation of pluripotent cells. Induced pluripotent stem (iPS) cell generation from somatic cells leads to the upregulation and downregulation of identified lncRNAs. The modulation of these lncRNAs are capable of enhancing pluripotency of somatic cells as well as enhancing differentiation of a pluripotent cell.Type: GrantFiled: June 27, 2014Date of Patent: September 13, 2016Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Daniel H. Kim, Barbara J. Wold
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Publication number: 20120053335Abstract: Microfluidic nucleic acid hybridization systems are described that include a first reaction chamber to hold an analyte solution comprising nucleic acids, and a first mixing channel in fluid communication with the chamber. The mixing channel includes a textured surface to mix the analyte solution. The systems may also include pump coupled to the mixing channel to circulate the analyte solution through the reaction chamber and the mixing channel, and an input port in fluid communication with the mixing channel and the reaction chamber to supply the analyte solution to the microfluidic system. The input port can be closed to create a closed circulation path for the analyte solution through the reaction chamber and the mixing channel.Type: ApplicationFiled: October 6, 2011Publication date: March 1, 2012Applicant: California Institute of TechnologyInventors: Jian Liu, Brian A. Williams, Barbara J. Wold, Stephen Quake
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Publication number: 20100197522Abstract: Microfluidic nucleic acid hybridization systems are described that include a first reaction chamber to hold an analyte solution comprising nucleic acids, and a first mixing channel in fluid communication with the chamber. The mixing channel includes a textured surface to mix the analyte solution. The systems may also include pump coupled to the mixing channel to circulate the analyte solution through the reaction chamber and the mixing channel, and an input port in fluid communication with the mixing channel and the reaction chamber to supply the analyte solution to the microfluidic system. The input port can be closed to create a closed circulation path for the analyte solution through the reaction chamber and the mixing channel.Type: ApplicationFiled: October 5, 2009Publication date: August 5, 2010Applicant: California Institute of TechnologyInventors: Jian Liu, Brian A. Williams, Barbara J. Wold, Stephen Quake
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Patent number: 6811977Abstract: The invention provides methods of identifying one or more nucleic acids in a sample. The nucleic acids, for example, expressed genes in a cell, can be identified by contacting the nucleic acids with oligonucleotides having detector tags, and selector tags to form tagged oligonucleotides. Each nucleic acid can be uniquely identified by mass-spectrophotometric analysis of the detector tag.Type: GrantFiled: July 27, 2001Date of Patent: November 2, 2004Assignee: California Institute of TechnologyInventors: Barbara J. Wold, John F. Murphy, Mark E. Davis, Kent Kirshenbaum, David A. Tirrell
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Publication number: 20040005704Abstract: The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described.Type: ApplicationFiled: June 13, 2003Publication date: January 8, 2004Applicants: California Institute of Technology, National Institutes of HealthInventors: Marie Csete, John Doyle, Barbara J. Wold, Ron McKay, Lorenz Studer
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Publication number: 20030207306Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.Type: ApplicationFiled: April 7, 2003Publication date: November 6, 2003Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventor: Barbara J. Wold
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Patent number: 6610540Abstract: The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described.Type: GrantFiled: October 22, 1999Date of Patent: August 26, 2003Assignees: California Institute of Technology, National Institutes of HealthInventors: Marie Csete, John Doyle, Barbara J. Wold, Ron McKay, Lorenz Studer
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Patent number: 6562567Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.Type: GrantFiled: January 27, 1999Date of Patent: May 13, 2003Assignee: California Institute of TechnologyInventor: Barbara J. Wold
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Patent number: 6544738Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.Type: GrantFiled: April 25, 2000Date of Patent: April 8, 2003Assignee: California Institute of TechnologyInventor: Barbara J. Wold
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Publication number: 20020137057Abstract: The invention provides methods of identifying one or more nucleic acids in a sample. The nucleic acids, for example, expressed genes in a cell, can be identified by contacting the nucleic acids with oligonucleotides having detector tags, and selector tags to form tagged oligonucleotides. Each nucleic acid can be uniquely identified by mass-spectrophotometric analysis of the detector tag.Type: ApplicationFiled: July 27, 2001Publication date: September 26, 2002Inventors: Barbara J. Wold, John F. Murphy, Mark E. Davis, Kent Kirshenbaum, David A. Tirrell
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Publication number: 20020098478Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kilts comprising oligonucleotides linked to a cleavable tag.Type: ApplicationFiled: January 27, 1999Publication date: July 25, 2002Inventor: BARBARA J. WOLD
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Publication number: 20020098482Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.Type: ApplicationFiled: April 25, 2000Publication date: July 25, 2002Inventor: Barbara J. Wold
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Patent number: 5599696Abstract: A method for amplifying specific nucleic acid fragments having an undefined nucleotide sequence wherein said nucleic acid fragments are present in a mixture of nucleic acids. Blunt ended linkers with known nucleotide sequences are used in the method to isolate and distinguish the specific undefined nucleic acid fragments from the rest of the nucleic acid. Primers which hybridize with the known linker are then used as part of a polymerase chain reaction procedure to amplify the specific nucleic acid fragment which includes the undefined nucleotide sequence.Type: GrantFiled: October 17, 1994Date of Patent: February 4, 1997Assignee: California Institute of TechnologyInventors: Paul R. Mueller, Paul A. Garrity, Barbara J. Wold