Abstract: A method of determining the identity and concentration of a target analyte present in a biological sample includes: introducing a first target analyte into a hollow core of an optical fiber; introducing a first reference calibrant into the hollow core of the optical fiber; transmitting light from a laser light source through the hollow core of the optical fiber and the first target analyte to generate a first Raman anti-Stokes analyte emission corresponding to the first target analyte; receiving the first Raman anti-Stokes analyte emission at a spectral analysis system optically coupled to the optical fiber; and deriving Raman anti-Stokes spectral peaks or spectra of the first target analyte at the spectral analysis system based on the first Raman anti-Stokes analyte emission.

Abstract: The present disclosure relates to compositions and methods useful for dietary augmentation to provide therapeutic intervention for the treatment and management of acne-prone skin. According to one embodiment of the present disclosure, there is provided an orally administrable composition containing a therapeutically effective amount of: (a) a probiotic blend of bacterial components containing at least: (i) Lactobacillus acidophilus, (ii) Bifidobacterium lactis, an (iii) Bacillus coagulans; (b) guggul; and (c) green tea leaf.

Abstract: The present disclosure relates to compositions and methods useful for dietary augmentation to provide therapeutic intervention for the treatment and management of acne-prone skin. According to one embodiment of the present disclosure, there is provided an orally administrable composition containing a therapeutically effective amount of: (a) a probiotic blend of bacterial components containing at least: (i) Lactobacillus acidophilus, (ii) Bifidobacterium lactis, an (iii) Bacillus coagulans; (b) guggul; and (c) green tea leaf.

Abstract: A method for enhancing mixing and aeration of a liquid reaction medium in a toroidal bioreactor vessel includes dispensing a liquid reaction medium into an interior of a toroidal bioreactor vessel, the interior being bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a plurality of upstanding protuberances. The toroidal bioreactor vessel is rotated in an orbital motion such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel.

Abstract: A method of carrying out a bioreaction in a multivessel bioreactor system may include flowing a culture medium at an initial flow rate through one or more fluidic paths between a master vessel and one or more slave vessels, detecting a first culture medium parameter value in the master vessel or the slave vessels, transmitting the first culture medium parameter value to a control system, determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value, and controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the one or more fluidic paths. The control system may adjust the flow rate of the culture medium to maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the one or more slave vessels.

Abstract: A method of manufacturing a composite sensor assembly includes: providing a port including a high surface tension thermoplastic, the port having a hollow tubular portion and a base having a polymer surface; fusing the polymer surface of the port to a wall of a polymeric bioprocess vessel so that the port communicates with an opening in the wall; and retaining a sensor unit within the hollow tubular portion of the port with an adhesive, at least a portion of the sensor unit being polymeric, wherein the port provides the sensor unit access to an interior of the polymeric bioprocess vessel.

Abstract: A method of carrying out a bioreaction in a multivessel bioreactor system may include flowing a culture medium at an initial flow rate through one or more fluidic paths between a master vessel and one or more slave vessels, detecting a first culture medium parameter value in the master vessel or the slave vessels, transmitting the first culture medium parameter value to a control system, determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value, and controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the one or more fluidic paths. The control system may adjust the flow rate of the culture medium to maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the one or more slave vessels.

Abstract: A method of manufacturing a composite sensor assembly includes: providing a port including a high surface tension thermoplastic, the port having a hollow tubular portion and a base having a polymer surface; fusing the polymer surface of the port to a wall of a polymeric bioprocess vessel so that the port communicates with an opening in the wall; and retaining a sensor unit within the hollow tubular portion of the port with an adhesive, at least a portion of the sensor unit being polymeric, wherein the port provides the sensor unit access to an interior of the polymeric bioprocess vessel.

Abstract: A decentralized distributed vaccine manufacturing systems and methods thereof provide a cost effective, simple to operate, automated, and small-scale development and manufacturing process by automated computer-controlled devices. The devices and methods disclosed that allows localized vaccine development and manufacture. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems.

Abstract: The present disclosure is directed to a composition and method of enhancing skin health and appearance, as well as treating compromised skin, by applying onto the skin a composition containing a moisturizing/hydrating-effective amount of a mixture of at least: (a) an oil infusion of Calendula officinalis extract; (b) an oil infusion of Helichrysum italicum extract; (c) an oil infusion of Symphytum officinale extract; (d) an aqueous infusion of Viola tricolor extract; and (e) an aqueous infusion of at least one Mallow extract chosen from Malva sylvestris extract and Althea officinalis extract; and an emulsifying-effective amount of a dermatologically-acceptable emulsifier, wherein the composition is natural and free of a skin sensitizing-effective amount of an essential oil.

Abstract: The present disclosure is directed to a composition for managing exaggerated sebum production in oily-prone skin, the composition containing: (1) a mixture of at least: (a) from about 0.02 to about 1% by weight of an ingredient capable of activating cannabinoid type-2 receptors (“CB2”); (b) from about 0.1 to about 3% by weight of a blend of Tetraselmis chuii and Fucus spiralis algae extracts; (c) from about 0.1 to about 4% by weight of an extract of Centella asiatica; and (d) from about 0.01 to about 0.05% by weight of an extract of Tulsi, wherein all weights are based on total weight of the composition; (2) optionally, an emulsifier; and (3) a dermatologically acceptable carrier, wherein the composition has a pH ranging from about 4.5 to about 5.5.

Abstract: A method of determining the identity and concentration of a target analyte present in a biological sample includes: introducing a first target analyte into a hollow core of an optical fiber; introducing a first reference calibrant into the hollow core of the optical fiber; transmitting light from a laser light source through the hollow core of the optical fiber and the first target analyte to generate a first Raman anti-Stokes analyte emission corresponding to the first target analyte; receiving the first Raman anti-Stokes analyte emission at a spectral analysis system optically coupled to the optical fiber; and deriving Raman anti-Stokes spectral peaks or spectra of the first target analyte at the spectral analysis system based on the first Raman anti-Stokes analyte emission.

Abstract: A method of carrying out a bioreaction in a multivessel bioreactor system may include flowing a culture medium at an initial flow rate through one or more fluidic paths between a master vessel and one or more slave vessels, detecting a first culture medium parameter value in the master vessel or the slave vessels, transmitting the first culture medium parameter value to a control system, determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value, and controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the one or more fluidic paths. The control system may adjust the flow rate of the culture medium to maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the one or more slave vessels.

Abstract: A bioreactor system may include (a) two or more fluidically coupled vessels configured to collectively carry out a bioreaction; (b) one or more fluidic paths coupling the two or more vessels to one another, where the fluidic paths are configured to provide flow of culture medium between the two or more vessels; (c) one or more fluid transfer devices along at least some of the one or more fluidic paths; and (d) a control system. The control system may be configured to (i) read or receive values of one or more parameters characterizing the culture medium in one or more of the vessels, and (ii) use the values to determine an adjusted flow rate in at least one of the fluidic paths to maintain substantially uniform values of the one or more parameters in the culture medium from vessel-to-vessel among the two or more vessels.

Abstract: A multi-analyte sensor system based on hollow core photonic bandgap fiber and Raman anti-Stokes spectroscopy. The system includes: i) an inlet to introduce an analyte sample into an analyzer chamber which analyzer includes; ii) a measurement system to derive the anti-Stokes spectral peaks and/or spectra of the sample; iii) a set of reference calibrants corresponding to the analytes of which the sample is primarily comprised; iv) a second inlet to introduce said calibrants into the analyzer chamber; v) a second measurement system to derive the anti-Stokes spectral peaks and/or spectra of the calibrants vi) an outlet through which the sample and calibrants are expelled from the analyzer chamber.

Abstract: A method of manufacturing a composite sensor assembly includes: providing a port including a high surface tension thermoplastic, the port having a hollow tubular portion and a base having a polymer surface; fusing the polymer surface of the port to a wall of a polymeric bioprocess vessel so that the port communicates with an opening in the wall; and retaining a sensor unit within the hollow tubular portion of the port with an adhesive, at least a portion of the sensor unit being polymeric, wherein the port provides the sensor unit access to an interior of the polymeric bioprocess vessel.

Abstract: Compositions intended for application onto human skin damaged by oxidative stress can include (1) a mixture of at least: (a) a leaf extract of Aristotelia chilensis; (b) a leaf extract of Buddleja globosa; (c) a leaf extract of Ugni molinae; (d) optionally, a bark/seed extract of Entada phaseoloides; (e) optionally, a blend of Pfaffia paniculata, Ptychopetalum olacoides, and Lilium candidum extracts; and (f) at least one humectant; (2) an emulsifier; and (3) a dermatologically acceptable carrier, wherein (a)-(f) are employed in amounts sufficient to synergistically neutralize existing free radicals present in the skin, while proactively priming the skin by repairing and/or protecting its Stratum corneum without unduly impairing the Stratum corneum's acid mantle, and wherein the composition is natural, free of a skin-sensitizing amount of an essential oil, and has a pH ranging from about 4.5 to about 5.5.

Abstract: The present disclosure is directed to a composition intended for application onto human skin suffering from oxidative stress, the composition comprising: (1) a mixture of at least: (a) a leaf extract of Aristotelia chilensis; (b) a leaf extract of Buddleja globosa; (c) a leaf extract of Ugni molinae; (d) optionally, a bark/seed extract of Entada phaseoloides; (e) optionally, a blend of Pfaffia paniculata, Ptychopetalum olacoides, and Lilium candidum extracts; and (f) at least one humectant; (2) an emulsifier; (3) a dermatologically acceptable carrier; and (4) a film former, wherein (a)-(f) are employed in amounts sufficient to neutralize existing free radicals present in the skin, while proactively priming the skin in order to enhance its ability to protect itself against free radical aggression caused by exposome-induced oxidative stress and dry skin-inducing water loss, wherein the composition is natural and free of a skin sensitizing-effective amount of an essential oil.

Abstract: A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

Abstract: The present disclosure is directed to a composition intended for application onto human skin suffering from oxidative stress, the composition comprising: (1) a mixture of at least: (a) a leaf extract of Aristotelia chilensis; (b) a leaf extract of Buddleja globosa; (c) a leaf extract of Ugni molinae; (d) optionally, a bark/seed extract of Entada phaseoloides; (e) optionally, a blend of Pfaffia paniculata, Ptychopetalum olacoides, and Lilium candidum extracts; and (f) at least one humectant; (2) an emulsifier; (3) a dermatologically acceptable carrier; and (4) a film former, wherein (a)-(f) are employed in amounts sufficient to neutralize existing free radicals present in the skin, while proactively priming the skin in order to enhance its ability to protect itself against free radical aggression caused by exposome-induced oxidative stress and dry skin-inducing water loss, wherein the composition is natural and free of a skin sensitizing-effective amount of an essential oil.