Abstract: Methods of screening a test agent for activity to modulate an oxidative demethylation enzyme are provided according to embodiments of the present invention which includes preparing a test reaction comprising an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme, a test agent and 4-amino-3-penten-2-one under conditions which allow generation of formaldehyde by oxidative demethylation of the substrate by the enzyme in the absence of the test agent; detecting fluorescence in the test reaction, the fluorescence indicative of reaction of formaldehyde and 4-amino-3-penten-2-one in the test reaction; and comparing fluorescence detected in the test reaction with a control, wherein a difference in detected fluorescence is indicative of a change in activity of the oxidative demethylation enzyme in the presence of the test agent.

Abstract: Methods of screening a test agent for activity to modulate an oxidative demethylation enzyme are provided according to embodiments of the present invention which includes preparing a test reaction comprising an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme, a test agent and 4-amino-3-penten-2-one under conditions which allow generation of formaldehyde by oxidative demethylation of the substrate by the enzyme in the absence of the test agent; detecting fluorescence in the test reaction, the fluorescence indicative of reaction of formaldehyde and 4-amino-3-penten-2-one in the test reaction; and comparing fluorescence detected in the test reaction with a control, wherein a difference in detected fluorescence is indicative of a change in activity of the oxidative demethylation enzyme in the presence of the test agent.

Abstract: Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Abstract: Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Abstract: Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Abstract: A device for protecting furniture from pet damage for preventing pets from chewing on covered areas of the furniture covered by the panels of the invention. The device for protecting furniture from pet damage includes a plurality of protective panels comprising side edges, end edges, faces and a connector system; and an anchoring panel comprising an anchor edge and T-shaped protrusions.