Abstract: Card game and method using a standard deck, and is played by a dealer and at least one player. Each hand includes dealing face-down three cards to the dealer, dealing face-down two cards to each player, permitting each player to view only a first dealt card, and requiring each player to make a decision about selection of a playing card after viewing the first dealt card which could be to stay with the first card which would thus constitute the player's playing card or to pass the first card and view the second card which would then constitute the player's playing card. Thereafter, all of the dealer's cards are uncovered and one of the dealer's cards is selected as the dealer's playing card, the assigned card being the one having the best value to achieve an objective of the game and method. Each player's playing card is revealed and compared to the dealer's playing card. A winner of the hand is determined based on the comparison.

Abstract: Cancer associated antigens have been identified by autologous antibody screening of libraries of nucleic acids expressed in testis cells using antisera from seminoma patients. The invention relates to nucleic acids and encoded polypeptides which are cancer associated antigens expressed in patients afflicted with a variety of cancers. The invention provides, inter alia, isolated nucleic acid molecules, expression vectors containing those molecules and host cells transfected with those molecules. The invention also provides isolated proteins and peptides, antibodies to those proteins and peptides and cytotoxic T lymphocytes which recognize the proteins and peptides. Fragments of the foregoing including functional fragments and variants also are provided. Kits containing the foregoing molecules additionally are provided. The molecules provided by the invention can be used in the diagnosis, monitoring, research, or treatment of conditions characterized by the expression of one or more cancer associated antigens.

Abstract: A process is provided for the purification of a proteinaceous physiologically active substance having antitumor activity, which is induced by administering to a rabbit at least one substance having a capacity for stimulating reticuloendothelial system and then injecting endotroxin from a Gram-negative bacterium into the rabbit. The process comprises contacting a crude solution of said proteinaceous physiologically active substance with a basic anion exchanger to have said physiologically active substance adsorbed on the anion exchanger, eluting the adsorbed physiologically active substance, and subjecting the eluate containing said physiologically active substance to gel filtration with a gel suitable for separation of a substance with a molecular weight in the range of 30,000 to 70,000. The purified preparation of said physiologically active substance thus obtained may be used as an antitumore agent for the treatment of malignant tumors.