Abstract: The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structures and the biological role of these cross-? structures. In one embodiment, the invention discloses a method for modulating extracellular protein degradation and/or protein clearance comprising modulating cross-?(beta) structure formation (and/or cross-? structure-mediated activity) of the protein present in the circulation.

Abstract: The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structures and the biological role of these cross-? structures. In one embodiment, the invention discloses a method for modulating extracellular protein degradation and/or protein clearance comprising modulating cross-?(beta) structure formation (and/or cross-? structure-mediated activity) of the protein present in the circulation.

Abstract: The invention relates to the detection and/or removal of conformationally altered proteins and/or molecules comprising a cross-? structure from a pharmaceutical composition. Disclosed is that unwanted and/or toxic side effects of pharmaceuticals are caused by proteins present in the pharmaceutical and adopting a cross-? structure conformation. Further disclosed is a method for detecting a protein in a pharmaceutical composition, the method comprising: contacting the pharmaceutical composition or any of its constituents comprising a protein with at least one cross-? structure-binding compound resulting in a bound protein and/or peptide comprising a cross-? structure and; detecting whether bound protein and/or peptide comprising a cross-? structure are present in the pharmaceutical composition or any of its constituents comprising a protein. Further described are methods for removing cross-? structures from a pharmaceutical composition and controlling the manufacture of a pharmaceutical composition.

Abstract: The invention relates to the field of biochemistry, biophysical chemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structure conformation. Even more particular, the invention relates to compounds capable of binding to a compound with cross-? structure conformation, i.e., cross-? structure binding compounds and uses thereof.

Abstract: The invention provides means and methods for producing and/or selecting immunogenic compositions, comprising providing said composition with at least one epitope for a B-cell receptor and/or at least one epitope for a T-cell receptor, coupled to a protein that comprises at least one crossbeta structure, and testing at least one immunogenic property.

Abstract: The invention provides means and methods for producing and/or selecting immunogenic compositions, comprising providing said composition with at least one crossbeta structure and testing at least one immunogenic property.

Abstract: The invention provides means and methods for producing and/or selecting immunogenic compositions capable of activating a T-cell and/or a T-cell response, comprising providing said composition with at least one crossbeta structure and testing at least one immunogenic property.

Abstract: The invention relates to the field of biochemistry, biophysical chemistry, molecular biology, structural biology, immunology, cellular biology and medicine. More in particular, the invention relates to the capability (or property) of chaperones to bind a crossbeta structure. Even more in particular, the invention relates to extracellular chaperones such as BiP, haptoglobin, hsp72 or clusterin. The present invention provides the insight that a chaperone molecule and more in specific an extra-cellular chaperone molecule (such as for example BiP, clusterin, hsp72 or haptoglobin) is capable of interacting with a crossbeta structure and/or a molecule comprising a crossbeta structure and/or a molecule comprising a crossbeta structure precursor. Based on this insight, the present inventors have developed multiple methods and means.

Abstract: The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structures and the biological role of these cross-? structures.

Abstract: Provided is means and methods for producing and/or selecting immunogenic compositions capable of activating a T-cell and/or a T-cell response, comprising providing the composition with at least one cross-beta structure and testing at least one immunogenic property.

Abstract: The present invention provides a method for selecting from a collection of IgIV molecules, at least one IgIV molecule comprising an affinity region that is capable of interacting with a misfolded protein and/or with an epitope of a cross-? structure and/or with an epitope of a protein comprising a cross-? structure, said method comprising contacting a collection of IgIV molecules with a misfolded protein and/or with a cross-? structure and/or with a protein comprising a cross-? structure and collecting at least one IgIV molecule comprising an affinity region interacting with said misfolded protein and/or epitope.

Abstract: Described are means and methods for producing and/or selecting immunogenic compositions, comprising providing the composition with at least one cross-beta structure and testing at least one immunogenic property.

Abstract: The invention relates to the field of biochemistry, biophysical chemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structure conformation. Even more particular, the invention relates to compounds capable of binding to a compound with cross-? structure conformation, i.e. cross-? structure binding compounds and uses thereof.

Abstract: The invention relates to the field of biochemistry, biophysical chemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structure conformation. The invention provides a method for determining a difference in the cross-? structure content of a protein in a reference sample compared to said protein in a test sample wherein the test sample has been subjected to a treatment that is expected to have an effect on the cross-? structure content of said protein comprising—determining in said reference sample the cross-? structure content of said protein—subjecting said protein to a treatment that is expected to have an effect on the cross-? structure content to obtain said test sample—determining in said obtained test sample the cross-? structure content of said protein—determining whether the cross-? structure content of the reference sample is different from the cross-? structure content in the test sample.

Abstract: The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-? structures and the biological role of these cross-? structures. In one embodiment, the invention discloses a method for modulating extracellular protein degradation and/or protein clearance comprising modulating cross-?(beta) structure formation (and/or cross-? structure-mediated activity) of the protein present in the circulation.

Abstract: The invention relates to the field of aqueous solutions comprising a protein. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or peptides comprising a cross-? structure from an aqueous solution comprising a protein. The invention provides methods for detecting and/or removing proteins and/or peptides comprising a cross-? structure from an aqueous solution comprising a protein, said method comprising contacting said aqueous solution comprising a protein with at least one cross-? structure-binding compound resulting in a bound protein or peptide with cross-? structure. The invention further provides a aqueous solution comprising a protein obtainable by a method of the invention, and a kit for carrying out the methods of the invention.

Abstract: The invention relates to the field of compositions comprising a protein, more specifically to pharmaceutical compositions. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or molecules comprising a cross-? structure from a pharmaceutical composition or any of its constituents comprising a protein. The present invention discloses that unwanted and/or toxic side effects of pharmaceuticals are caused by proteins present in said pharmaceutical and adopting a cross-b structure conformation.

Abstract: The invention relates to novel methods and means for providing proteinaceous substances, such as peptides, polypeptides, glycoproteins, lipoproteins and complex compounds comprising the former in combination with other substances, such as nucleic acids, membrane structures, carbohydrate structures, with cross-? structures, which enhance the immunogenicity of said proteinaceous substance. The resulting peptides, proteins, glycoproteins, etc. are preferably used in vaccines. The invention provides a method for producing an immunogenic composition comprising at least one peptide, polypeptide, protein, glycoprotein and/or lipoprotein, comprising providing said composition with at least one cross-? structure. The invention also discloses the use of cross-? structures in the preparation of a vaccine for the prophylaxis of an infectious disease.

Abstract: The invention relates to the detection and/or removal of conformationally altered proteins and/or molecules comprising a cross-? structure from a pharmaceutical composition or any of its constituents comprising a protein. Disclosed iss that unwanted and/or toxic side effects of pharmaceuticals are caused by proteins present in the pharmaceutical and adopting a cross-? structure conformation. Further disclosed is a method for detecting a protein and/or peptide comprising a cross-? structure in a pharmaceutical composition or any of its constituents comprising a protein, the method comprising: contacting the pharmaceutical composition or any of its constituents comprising a protein with at least one cross-? structure-binding compound resulting in a bound protein and/or peptide comprising a cross-? structure and; detecting whether bound protein and/or peptide comprising a cross-? structure are present in the pharmaceutical composition or any of its constituents comprising a protein.

Abstract: The invention relates to the field of aqueous solutions comprising a protein. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or peptides comprising a cross-? structure from an aqueous solution comprising a protein. The invention provides methods for detecting and/or removing proteins and/or peptides comprising a cross-? structure from an aqueous solution comprising a protein, the method comprising contacting the aqueous solution comprising a protein with at least one cross-? structure-binding compound resulting in a bound protein or peptide with cross-? structure. The invention further provides a aqueous solution comprising a protein obtainable by a method of the invention, and a kit for carrying out the methods of the invention.