Abstract: Provided herein are systems and methods for the enrichment and detection of nucleic acid molecules. In particular, provided herein are systems and methods for the selective enrichment of a desired nucleic acid molecule in a sample containing non-desired nucleic acid molecules in which a probe with different complementarity to the the desired compared to the undesired nucleic acid, e.g. wild-type and variant. The probe may be bound to the solid phase, e.g. via biotin, the probe binding to the undesired may be cleaved so that the undesired is released and the desired nucleic acid is enriched by binding to the solid phase. The method may also employ differential release from the probe.

Abstract: Provided herein are compositions and methods for the analysis of nucleic acids. In particular, provided herein are compositions and methods employing differential complementarity of probe nucleic acid molecules to target nucleic acids molecules to selectively isolate and detect targets or probes as an indication of the presence of and/or amount of a particular target nucleic acid molecule in a sample.

Abstract: Provided herein are methods for improved polynucleotide detection.

Abstract: Provided herein are methods, kits and devices which may be used for the detection of epigenetic modifications.

Abstract: Provided herein are methods for improved polynucleotide detection.

Abstract: Disclosed is an improved polynucleotide sequence detection method suitable for testing for the presence of a large number of diagnostic markers, including those used in the identification of cancer, infectious disease and transplant organ rejection.

Abstract: This invention relates to a method of detecting two or more target polynucleotide sequences comprising using a probe oligonucleotide A0 annealing to the target, whereafter the 3? end is pyrophosphorolysed and the remaining probe is circularised or ligated to another probe using a splint oligonucleotide, and a padlock probe X0, which is circularised against the target and not pyrophosphorolysed, and then a signal from (amplicons of) the ligated probes is detected to indicate presence of the target. Further disclosed are kits comprising the probes and enzymes as well as buffer and a source of pyrophosphate ions.

Abstract: This invention relates to simplified polynucleotide sequence detection methods suitable for testing for the presence of a large number of diagnostic markers, including those used in the identification of cancer, infectious disease and transplant organ rejection. It is also useful for companion diagnostic testing in which a panel of markers must be identified reliably and at low cost.

Abstract: This invention relates to molecular systems for detecting a target polynucleotide sequence in a given nucleic acid analyte and methods of use thereof.

Abstract: Provided herein are kits and devices which may be used for improved polynucleotide detection.