Abstract: Provided are methods, compositions, and kits for improving a delivery of a nucleic acid to a cell in an organ of a subject. Such methods, compositions, and kits may include a sonoactive agent and a nucleic acid comprising a cargo polynucleotide and a nuclear localization element and/or an innate immune response avoidance moiety, which may be an aptamer. Delivery may involve sonoporation.

Abstract: Techniques are disclosed for volume promotion management and visualization in a metro cluster. For example, a system can include a processor coupled to a memory, the processor configured to: receive a promotion request directed to a selected volume of a cluster volume, wherein the cluster volume is established between a first volume in a first array and a second volume in a second array, the selected volume being one of the first and second volume; collect metrics that indicate respective characteristics associated with the cluster volume; and in response to receiving the request: compare the metrics with a database that associates permutations of metrics with respective conditions for promoting the selected volume, determine, based on the comparison, a target condition set having a permutation of metrics that matches the metrics, and perform a promotion procedure on the selected volume upon a determination that the target condition set is satisfied.

Abstract: Techniques are disclosed for volume promotion management and visualization in a metro cluster. For example, a system can include a processor coupled to a memory, the processor configured to: receive a promotion request directed to a selected volume of a cluster volume, wherein the cluster volume is established between a first volume in a first array and a second volume in a second array, the selected volume being one of the first and second volume; collect metrics that indicate respective characteristics associated with the cluster volume; and in response to receiving the request: compare the metrics with a database that associates permutations of metrics with respective conditions for promoting the selected volume, determine, based on the comparison, a target condition set having a permutation of metrics that matches the metrics, and perform a promotion procedure on the selected volume upon a determination that the target condition set is satisfied.

Abstract: A holder assembly is described for releasably receiving and supporting successive groups of containers in a case packing machine. The machine is operable to move successive groups vertically downward from a first station to the holder assembly at a second station. Subsequently the containers are forced downwardly again from the holder assembly to a case packing station below. The holder assembly functions to group received containers into defined rectangular clusters. The holder assembly also permits vertical movement of the containers while holding them in the rectangular array. Rotary mechanisms enable selective inversion of the holders and containers supported within.

Abstract: A case packing machine is described for bottles having enlarged neck flanges. The machine first arranges bottles received from a conveyor into a group on an escapement mechanism. The grouped bottles are suspended from their enlarged neck flanges by an escapement mechanism at a first station. The group is released as a ram engages the bottle finishes and shifts them downwardly to a second station. An actuator on the ram functions to operate the escapement mechanism and a bottle stop mechanism is also operated directly from the ram to halt further progress of bottles toward the escapement mechanism during release and movement of the previous group of bottles to the second station. Bottles may be received by an invertible holder at the second station. The holder forms the bottles into a prescribed rectangular array and supports the bottles until a subsequent group is received. The subsequent received group engages and moves the first group downwardly into a case waiting at a packing station below.