Abstract: Methods for the separation of biological materials from a sample mixture using a superficially porous particle coated (or clad) with a metal are provided herein. Methods using the support as a chromatographic column are also provided.

Abstract: Apparatus and methods are disclosed for digesting a biopolymer in a medium. The medium is disposed adjacent a wall of a hollow element wherein at least a portion of the wall is porous. The medium is then exposed to digestion conditions to cleave the biopolymer into fragments. The fragments are permitted to permeate through the wall of the hollow element. In some embodiments a surface of a wall of the hollow element comprises a digestion agent. In some embodiments the hollow element is disposed within a hollow liner. For analysis and identification purposes, the fragments may be separated and/or detected.

Abstract: Serial derivatization by chemical reactions of analytes for mass spectrometry is disclosed. The derivatizations enhance the use of MS techniques for analyzing protein samples, particularly when the sequence of a polypeptide is determined by tandem MS/MS. Accurate mass analysis techniques are described for use in sequencing polypeptides, together with the use of sequencing data in protein analysis.