Abstract: Reagent devices including a liquid container and a reagent insert are provided. Aspects of the reagent insert include a first dried reagent composition and a second dried reagent composition distinctly positioned on one or more surfaces of a solid support. Also provided are methods of making and using the reagent device or reagent insert, as well as systems and kits including the same.

Abstract: Containers including one or more dried reagent compositions are provided. Aspects a subject container may include a container bottom portion defining a first volume and comprising a first dried reagent composition positioned on an inner surface thereof, and a container wall portion attached to the container bottom portion at a sealed attachment region joining the container bottom portion to the container wall portion. Also provided are methods of making and using the container as well as kits including the same.