Abstract: Nodulisporic acids (NAs) comprise a group of indole diterpenes known for their potent insecticidal activities; however, biosynthesis of NAs by its natural producer, Hypoxylon pulicicidum (Nodulisporium sp.) is exceptionally difficult to achieve. The identification of genes responsible for NA production could enable biosynthetic pathway optimization to provide access to NAs for commercial applications. Obtaining useful quantities of NAs using published fermentations methods is challenging, making gene knockout studies an undesirable method to confirm gene function. Alternatively, heterologous gene expression of H. pulicicidum genes in a more robust host species like Penicillium paxilli provides a way to rapidly identify the function of genes that play a role in NA biosynthesis. In this work, we identified the function of four secondary-metabolic genes necessary for the biosynthesis of nodulisporic acid F (NAF) and reconstituted these genes in the genome of P.

Abstract: This disclosure describes a slide comprising a substrate, a wireless communication element, and a sensing element, wherein the slide is capable of communicating with an external communications device via the wireless communication element. In some implementations, the wireless communication element comprises an antenna, an integrated circuit, and a memory unit, that are powered wirelessly by an external device, such that it is capable of measuring, logging, and communicating data. The data, such as time and temperature, may be collected via the sensing element connected to the wireless communication element.

Abstract: This disclosure describes a slide comprising a substrate, a WISP, and a temperature sensor, wherein the slide is capable of communicating with an external communications device via the WISP. In some implementations, the WISP comprises an antenna, an integrated circuit, and a memory unit, that are powered wirelessly by an external device, such that it is capable of measuring, logging, and communicating data. The data, such as time and temperature, may be collected via sensors connected to the WISP. In some implementations, the WISP and temperature sensor are embedded in the substrate to prevent direct contact with mounted specimens. In some implementations the slide further comprises displayed content. In some implementations, the displayed content is linked to a database of information. The various implementations of this invention may describe a slide utilizing any number of the features described above in any combination.

Abstract: This disclosure describes a slide comprising a substrate, a WISP, and a temperature sensor, wherein the slide is capable of communicating with an external communications device via the WISP. In some implementations, the WISP comprises an antenna, an integrated circuit, and a memory unit, that are powered wirelessly by an external device, such that it is capable of measuring, logging, and communicating data. The data, such as time and temperature, may be collected via sensors connected to the WISP. In some implementations, the WISP and temperature sensor are embedded in the substrate to prevent direct contact with mounted specimens. In some implementations the slide further comprises displayed content. In some implementations, the displayed content is linked to a database of information. The various implementations of this invention may describe a slide utilizing any number of the features described above in any combination.

Abstract: This disclosure describes a slide comprising a substrate, a RFID or WISP, and a temperature sensor, wherein the slide is capable of communicating with an external communications device via the RFID or WISP. In some implementations, the RFID or WISP of the slide comprises an antenna, an integrated circuit, a memory unit, and a power source, such that it is capable of measuring and logging data related to time and temperature. In some implementations, the RFID or WISP and temperature sensor are embedded in the substrate to prevent direct contact with mounted specimens. In some implementations the slide further comprises displayed content. In some implementations, the displayed content is linked to a database of information. The various implementations of this invention may describe a slide utilizing any number of the features described above in any combination.

Abstract: This disclosure describes an enhanced temperature validation slide comprising a substrate and a removable label attached to the substrate with an adhesive. In some implementations, the label comprises at least two threshold temperature indicators, a transparent liquid impermeable barrier layer, a designated area for user generated information, an area for displayed information (i.e. a lot number), an area for displayed text or graphics linked to information stored online (i.e. a QR code), and a thermo-chromic visual indicator area. The various implementations of this disclosure may describe a validation slide utilizing any number of the features described above in any combination.

Abstract: This disclosure describes a slide comprising a substrate, a RFID or WISP, and a temperature sensor, wherein the slide is capable of communicating with an external communications device via the RFID or WISP. In some implementations, the RFID or WISP of the slide comprises an antenna, an integrated circuit, a memory unit, and a power source, such that it is capable of measuring and logging data related to time and temperature. In some implementations, the RFID or WISP and temperature sensor are embedded in the substrate to prevent direct contact with mounted specimens. In some implementations the slide further comprises displayed content. In some implementations, the displayed content is linked to a database of information. The various implementations of this invention may describe a slide utilizing any number of the features described above in any combination.

Abstract: This disclosure describes an enhanced temperature validation slide comprising a substrate and a removable label attached to the substrate with an adhesive. In some implementations, the label comprises at least two threshold temperature indicators, a transparent liquid impermeable barrier layer, a designated area for user generated information, an area for displayed information (i.e. a lot number), an area for displayed text or graphics linked to information stored online (i.e. a QR code), and a thermo-chromic visual indicator area. The various implementations of this disclosure may describe a validation slide utilizing any number of the features described above in any combination.

Abstract: A downhole apparatus and method for maintaining or reducing the level of liquids at the bottom of a gas producing well is described including a constriction or throat section, such as a Venturi, in which a production gas flow from the well is used to generate a low pressure zone having a pressure less that the ambient formation gas pressure and at least one conduit providing a flow path from an liquids at the bottom of said well to said low pressure zone. The conduit may be adaptable in length to provide for maintaining the flow path as the level of the liquids changes.

Abstract: A downhole apparatus and method for maintaining or reducing the level of liquids at the bottom of a gas producing well is described including a constriction or throat section, such as a Venturi, in which a production gas flow from the well is used to generate a low pressure zone having a pressure less that the ambient formation gas pressure and at least one conduit providing a flow path from an liquids at the bottom of said well to said low pressure zone. The conduit may be adaptable in length to provide for maintaining the flow path as the level of the liquids changes.

Abstract: A downhole apparatus and method for maintaining or reducing the level of liquids at the bottom of a gas producing well is described including a constriction or throat section, such as a Venturi, in which a production gas flow from the well is used to generate a low pressure zone having a pressure less that the ambient formation gas pressure and at least one conduit providing a flow path from an up-stream location within said well to said low pressure zone. The conduit may have additional opening for production gas to enter the conduit.

Abstract: A downhole apparatus and method for maintaining or reducing the level of liquids at the bottom of a gas producing well is described including a constriction or throat section, such as a Venturi, in which a production gas flow from the well is used to generate a low pressure zone having a pressure less that the ambient formation gas pressure and at least one conduit providing a flow path from an up-stream location within said well to said low pressure zone. The conduit may have additional opening for production gas to enter the conduit.

Abstract: The present invention relates to a method of regulating the flow rate of formation fluids produced from a determined zone of an underground well whose cased wall is provided with orifices through which said formation fluids can pass, said method consisting in applying a tubular structure along the casing in said zone, which tubular structure prevents the fluids from flowing directly while also preserving a flow path along which the fluids can flow via the annular space outside the tubular structure so as to generate head loss. The invention also relates to a device for implementing said method, which device is essentially constituted by a radially-expandable tubular structure that can be applied against the inside wall of the casing, the structure being provided with means for preserving the flow of the fluids via a path running along the casing and along the structure, in order to generate head loss.

Abstract: The present invention relates to a method of regulating the flow rate of formation fluids produced from a determined zone of an underground well whose cased wall is provided with orifices through which said formation fluids can pass, said method consisting in applying a tubular structure along the casing in said zone, which tubular structure prevents the fluids from flowing directly while also preserving a flow path along which the fluids can flow via the annular space outside the tubular structure so as to generate head loss. The invention also relates to a device for implementing said method, which device is essentially constituted by a radially-expandable tubular structure that can be applied against the inside wall of the casing, the structure being provided with means for preserving the flow of the fluids via a path running along the casing and along the structure, in order to generate head loss.

Abstract: A blanket includes a cavity therewithin defined between spaced layers of the blanket directing a fluid conduit therethrough. The fluid conduit is in cooperative and fluid communication with a control housing to include cooling and heating structure in association with a pump to direct cooled or heating fluid through the conduit within the blanket cavity. A modification of the invention includes a secondary reservoir to include fluid at ambient air temperature to stabilize a blanket temperature as an alternative to creating a temperature gradient relative to the ambient temperature environment of the blanket.