Patents by Inventor Bart Holmquist

Bart Holmquist has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
    Patent number: 6410707
    Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
    Type: Grant
    Filed: January 2, 2001
    Date of Patent: June 25, 2002
    Assignee: BioNebraska, Inc.
    Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
  • Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
    Publication number: 20010031856
    Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
    Type: Application
    Filed: January 2, 2001
    Publication date: October 18, 2001
    Applicant: Bio Nebraska, Inc.
    Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
  • Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
    Patent number: 6251635
    Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
    Type: Grant
    Filed: August 25, 1998
    Date of Patent: June 26, 2001
    Assignee: Bionebraska, Inc.
    Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
  • Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
    Patent number: 5962270
    Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
    Type: Grant
    Filed: February 6, 1996
    Date of Patent: October 5, 1999
    Assignee: Bionebraska, Inc.
    Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
  • Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof
    Patent number: 5656456
    Abstract: The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid .alpha.-carbon reactive group selected from the group consisting of N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid .alpha.-carbon reactive group.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: August 12, 1997
    Assignee: BioNebraska, Inc.
    Inventors: Jay Stout, Fred W. Wagner, Thomas R. Coolidge, Bart Holmquist
  • Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof
    Patent number: 5635371
    Abstract: The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid .alpha.-carbon reactive group selected from the group consisting of N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid .alpha.-carbon reactive group.
    Type: Grant
    Filed: August 23, 1994
    Date of Patent: June 3, 1997
    Assignee: BioNebraska, Inc.
    Inventors: Jay Stout, Fred W. Wagner, Thomas R. Coolidge, Bart Holmquist