Abstract: A focusing distance (e.g., a distance between a focal plane of a camera used when n image was captured and the actual focal plane for an in-focus image) in a visual analysis system may be determined by subjecting one or more images captured by such a system to a multi-layer analysis. In such an analysis, an input may be subjected to one or more convolution filters, and the ultimate result of such convolution may be provided to a dense layer which can provide the focusing distance. This focusing distance may then be used to (re) focus a camera or for other purposes (e.g., generating an alert).

Abstract: A focusing distance (e.g., a distance between a focal plane of a camera used when n image was captured and the actual focal plane for an in-focus image) in a visual analysis system may be determined by subjecting one or more images captured by such a system to a multi-layer analysis. In such an analysis, an input may be subjected to one or more convolution filters, and the ultimate result of such convolution may be provided to a dense layer which can provide the focusing distance. This focusing distance may then be used to (re)focus a camera or for other purposes (e.g., generating an alert).

Abstract: A sampling system that includes a traverse, a sample aspiration module body, and a mechanical system. The sample aspiration module body is configured to travel along the traverse. The mechanical system is configured to move the traverse from a first position to a second position, wherein both the first position and the second position lie in the same plane.

Abstract: A biological imaging analyzer is described comprises a staining module configured to stain cells of a biological sample so as to produce stained cells. The analyzer also comprises a lighting module configured to illuminate the stained cells, the lighting module comprising a plurality of pulsed lights. The analyzer further comprises an imaging module configured to capture images of the stained cells. A method of flow imaging a biological sample comprises flowing the biological sample including the stained cells through an image capture region of a flowcell. The method also comprises utilizing the lighting module to illuminate the stained cells at the image capture region with the plurality of pulsed lights. The method further comprises capturing images of the stained cells at the image capture region with the imaging module.

Abstract: An analyzer may have its operation validated using control samples which comprise synthetic particles. Such an analyzer be adapted to obtain a representation of a particle from a sample and obtain a classification of the particle which classifies the particle as a type specific to control samples. The analyzer may also be adapted to perform control specific processing on the sample, and to generate an analysis output for the sample based on a result of the control specific processing.

Abstract: A focusing distance (e.g., a distance between a focal plane of a camera used when n image was captured and the actual focal plane for an in-focus image) in a visual analysis system may be determined by subjecting one or more images captured by such a system to a multi-layer analysis. In such an analysis, an input may be subjected to one or more convolution filters, and the ultimate result of such convolution may be provided to a dense layer which can provide the focusing distance. This focusing distance may then be used to (re)focus a camera or for other purposes (e.g., generating an alert).

Abstract: A biological analysis system includes a probe. The probe is configured to dispense a biological sample into a first chamber. The system also includes a pump. The pump is configured to convey a first delivery of diluent to the first chamber for mixing with the biological sample to produce a sample mixture, aspirate a portion of the sample mixture from the first chamber, and convey the portion of the sample mixture to a second chamber. A method of analyzing a biological sample includes conveying a diluent to a first chamber via a first pump, dispensing a blood sample into the first chamber via a probe to produce a sample mixture, and conveying a portion of the sample mixture from the first chamber to a second chamber via the first pump.

Abstract: A biological analysis system includes a flow generator, a flowcell, a first flow path, and a second flow path. The flow generator is configured to provide a sheath fluid. The flowcell is configured to receive a biological sample. The first flow path is in fluid communication with the flow generator and configured to receive a first portion of the sheath fluid from the flow generator. The first flow path is configured to convey the biological sample using the first portion to the flowcell at a first flow rate. The second flow path is in fluid communication with the flow generator and configured to receive a second portion of the sheath fluid from the flow generator. The second flow path is configured to convey the second portion to the flowcell at a second flow rate. The second flow rate is different than the first flow rate.

Abstract: A biological imaging analyzer comprises a flowcell configured to flow biological cells therethrough, the flowcell including an imaging region where images of the biological cells are captured. The analyzer also comprises a lighting module configured to generate a light, the lighting module comprising a light guide configured to convey the light to an imaging region of the flowcell. The analyzer further comprises an imaging module configured to capture images of the biological cells at the imaging region of the flowcell. The analyzer also comprises a flowcell holder in operable connection with the flowcell. A method of positioning a flowcell for biological analysis comprises providing the flowcell configured to flow a biological sample for analysis, and operably connecting the flowcell holder with the flowcell.

Abstract: A biological imaging analyzer comprises a staining module configured to produce stained cells. The staining module comprises one or more chambers configured to receive a biological sample and a staining composition, and a heater configured to heat the biological sample and the staining composition. The analyzer also comprises a lighting module configured to illuminate the stained cells, and an imaging module configured to capture images of the stained cells. A method of staining a biological sample comprises depositing the staining composition into the one or more chambers, and pre-heating the staining composition in the one or more chambers. The method also comprises depositing the biological sample into the one or more chambers, and heating the biological sample and the staining composition in the one or more chambers.

Abstract: A biological imaging analyzer is described comprises a staining module configured to stain cells of a biological sample so as to produce stained cells. The analyzer also comprises a lighting module configured to illuminate the stained cells, the lighting module comprising a plurality of pulsed lights. The analyzer further comprises an imaging module configured to capture images of the stained cells. A method of flow imaging a biological sample comprises flowing the biological sample including the stained cells through an image capture region of a flowcell. The method also comprises utilizing the lighting module to illuminate the stained cells at the image capture region with the plurality of pulsed lights. The method further comprises capturing images of the stained cells at the image capture region with the imaging module.

Abstract: A sampling system that includes a traverse, a sample aspiration module body, and a mechanical system. The sample aspiration module body is configured to travel along the traverse. The mechanical system is configured to move the traverse from a first position to a second position, wherein both the first position and the second position lie in the same plane.

Abstract: Techniques for the sensitive detection of analytes which combine the benefits of solution/suspension phase assay formats and the simplicity of solid phase/lateral flow assays are described. The assays can be performed in the solution/suspension phase using magnetic microspheres as a solid support. Subsequently a magnetic separation can be performed to separate the bound analyte from the remainder of the solution. After a wash step, the fluorescence signal can be directly read from the magnetic particle surface. Portable biodetection systems which employ fluorescent polymer superquenching and methods for detecting bioagents therewith are also described.

Abstract: High throughput electrophoresis having a plurality of samples migrating through a highly resistive separatory media under an electric field. Multiple electrophoresis runs are conducted using a single separatory media. Automated methods of data analysis yields quantitative and qualitative data using optical densitometer or other detection systems. Controlled operating temperatures and small geometry reduces dispersion inaccuracy in banded data.