Patents by Inventor Bart Willem Swinkels

Bart Willem Swinkels has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Selection marker gene free recombinant strains
    Patent number: 6955909
    Abstract: The present invention discloses a selection marker free system which can be used to introduce genetic modifications to in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
    Type: Grant
    Filed: February 26, 1999
    Date of Patent: October 18, 2005
    Assignee: DSM N.V.
    Inventors: Gerardus Cornelis Maria Selten, Bart Willem Swinkels, Robertus Franciscus Maria Van Gorcom
  • Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
    Patent number: 6432672
    Abstract: The present invention relates to filamentous fungi that comprise in their genomes at least two substantially homologous DNA domains which are suitable for integration of one or more copies of a recombinant DNA molecule and wherein at least two of these DNA domains comprise an integrated copy of a recombinant DNA molecule. The invention also relates to methods for preparing such filamentous fungi and for further multiplying the DNA domains with integrated recombinant DNA molecules through gene conversion or amplification.
    Type: Grant
    Filed: January 24, 2000
    Date of Patent: August 13, 2002
    Inventors: Gerardus Cornelis Maria Selten, Bart Willem Swinkels, Roelof Ary Lans Bovenberg
  • Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
    Patent number: 6265186
    Abstract: The present invention provides for a yeast cell comprising at least two copies of a desired gene integrated into its chromosomal genome, wherein said genome comprises at least two DNA domains suitable for integration of one or more copies of said desired gene, which domains share substantial sequence homology and are non-ribosomal RNA encoding DNA domains, and wherein at least two of said substantially homologous non-ribosomal RNA encoding DNA domains have at least one copy of the said desired gene integrated. The invention also provides methods for making yeast cells according to the invention, as well as the use thereof for making a protein, a peptide or a metabolite.
    Type: Grant
    Filed: December 10, 1999
    Date of Patent: July 24, 2001
    Assignee: DSM N.V.
    Inventors: Bart Willem Swinkels, Albert Johannes Joseph Van Ooijen, Adriana Cornelia Maria Noordermeer-Van Der Haak
  • Transformed fungal strains free of selection marker
    Patent number: 6063614
    Abstract: The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
    Type: Grant
    Filed: November 10, 1998
    Date of Patent: May 16, 2000
    Assignee: DSM N.V.
    Inventors: Gerardus Cornelius Maria Selten, Bart Willem Swinkels, Robertus Franciscus Maria Van Gorcom
  • Selection marker gene free recombinant strains: a method for obtaining them and the use of these strains
    Patent number: 6051431
    Abstract: The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
    Type: Grant
    Filed: December 8, 1997
    Date of Patent: April 18, 2000
    Assignee: DSM N.V.
    Inventors: Gerardus Cornelius Maria Selten, Bart Willem Swinkels, Robertus Franciscus Maria Van Gorcom
  • Selection marker gene free recombinant strains: method for obtaining them and the use of these strains
    Patent number: 5876988
    Abstract: The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
    Type: Grant
    Filed: June 23, 1997
    Date of Patent: March 2, 1999
    Assignee: Gist-brocades BV
    Inventors: Gerardus Cornelius Maria Selten, Bart Willem Swinkels, Robertus Franciscus Maria Van Gorcom