Patents by Inventor Bartlomiej P. Rajwa
Bartlomiej P. Rajwa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230101936Abstract: The invention generally relates to methods, reagents, and substrates for detecting target analytes, especially spectroscopic techniques such as laser-induced breakdown spectroscopy (LIBS) for use in food authentication and molecular detection (e.g., when combined with later flow immunoassays (LFIA).Type: ApplicationFiled: September 27, 2022Publication date: March 30, 2023Inventors: Bartlomiej P. Rajwa, Euiwon Bae, J. Paul Robinson, Carmen Gondhalekar, Xi Wu
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Patent number: 11227142Abstract: A system for the characterization of a colony of microorganisms includes a coherent light source configured to provide coherent light of one or more wavelengths along a common optical path. A holder is configured to operationally arrange a substrate so that the colony of microorganisms on a surface of the substrate is positioned to receive the coherent light. Scattered light is generated from the colony of microorganisms receiving coherent light. A first image capture device is configured to receive the scattered light and generate a scatter image from the microorganism colony irradiated by the coherent light. The system also includes a magnifying lens configured to magnify the colony of microorganisms. A second image capture device is configured to capture a light image of the colony of microorganisms magnified by the magnifying lens. Methods of assigning organisms to categories with like organisms without necessarily identifying the organisms are also described.Type: GrantFiled: June 12, 2020Date of Patent: January 18, 2022Assignee: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Kathryn E. Ragheb, Cheryl M. Holdman, Valery P. Patsekin, Euiwon Bae, Jennifer Sturgis
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Publication number: 20210142037Abstract: A system for the characterization of a colony of microorganisms includes a coherent light source configured to provide coherent light of one or more wavelengths along a common optical path. A holder is configured to operationally arrange a substrate so that the colony of microorganisms on a surface of the substrate is positioned to receive the coherent light. Scattered light is generated from the colony of microorganisms receiving coherent light. A first image capture device is configured to receive the scattered light and generate a scatter image from the microorganism colony irradiated by the coherent light. The system also includes a magnifying lens configured to magnify the colony of microorganisms. A second image capture device is configured to capture a light image of the colony of microorganisms magnified by the magnifying lens. Methods of assigning organisms to categories with like organisms without necessarily identifying the organisms are also described.Type: ApplicationFiled: June 12, 2020Publication date: May 13, 2021Applicant: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Kathryn E. Ragheb, Cheryl M. Holdman, Valery P. Patsekin, Euiwon Bae, Jennifer Sturgis
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Patent number: 10769409Abstract: A computer method for correlating depictions of colonies of microorganisms includes receiving an image of a substrate associated with a first time and showing a colony of microorganisms. A second image of the same substrate and associated with a second time shows a candidate colony of microorganisms. A region of the second image that shows the candidate colony of microorganisms is located. The first region of the first image is compared to the second region of the second image. Based on the comparison of the images, the candidate colony of microorganism is determined to be the same colony as the first colony of microorganisms. Systems for moving substrates having colonies of microorganisms and maintaining orientation of the substrates before and after movement are also described.Type: GrantFiled: April 21, 2016Date of Patent: September 8, 2020Assignee: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery Patsekin
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Patent number: 10719691Abstract: A system for the characterization of a colony of microorganisms includes a coherent light source configured to provide coherent light of one or more wavelengths along a common optical path. A holder is configured to operationally arrange a substrate so that the colony of microorganisms on a surface of the substrate is positioned to receive the coherent light. Scattered light is generated from the colony of microorganisms receiving coherent light. A first image capture device is configured to receive the scattered light and generate a scatter image from the microorganism colony irradiated by the coherent light. The system also includes a magnifying lens configured to magnify the colony of microorganisms. A second image capture device is configured to capture a light image of the colony of microorganisms magnified by the magnifying lens. Methods of assigning organisms to categories with like organisms without necessarily identifying the organisms are also described.Type: GrantFiled: April 21, 2016Date of Patent: July 21, 2020Assignee: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Kathryn E. Ragheb, Cheryl M. Holdman, Valery P. Patsekin, Euiwon Bae, Jennifer Sturgis
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Publication number: 20200141873Abstract: Various techniques for characterizing a target within a sample are described. An example method includes applying, to the sample, a recognition construct that includes a metal and a scaffold, wherein the scaffold is configured to bind to the target. Energy can be applied to the sample, wherein the applied energy is sufficient to transform at least some of the sample into a plasma. Electromagnetic radiation emitted by the plasma can be detected to provide an optical-spectrum signal of the sample.Type: ApplicationFiled: December 20, 2019Publication date: May 7, 2020Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Publication number: 20200049629Abstract: An apparatus and method for characterizing a target, e.g., microbial samples or biological toxins, includes labeling the target with a biomolecular recognition construct and measuring an atomic-spectra signal of the biomolecular recognition construct. The method can include heating the labeled target before measuring the atomic-spectra signal. The atomic-spectra signal can be measured by performing laser-induced breakdown spectroscopy. The atomic-spectra signal can be measured by performing spark induced breakdown spectroscopy. The biomolecular recognition construct can be prepared by tagging a biological scaffolding with a metal atom or ion. In an aspect in which the target includes a microbial sample, the biological scaffolding can include an antibody against epitopes present on bacterial surface, the antibody linked to a heavy metal. In an aspect in which the target includes a biological toxin, the biological scaffolding can include an antibody against the biological toxin linked to heavy metals.Type: ApplicationFiled: October 16, 2019Publication date: February 13, 2020Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Patent number: 10514338Abstract: A target within a sample can be characterized using an energy source configured to transform a metal in the sample into a plasma and an optical spectroscopic detector configured to detect electromagnetic radiation emitted by the plasma to provide an optical-spectrum signal. A processor can determine presence of the metal in the sample using the optical-spectrum signal. The target can include a microbe or biological toxin. A recognition construct comprising a metal and a scaffold can be applied to the sample. The scaffold can bind to the target. Energy can be applied to transform at least some of the sample into a plasma. Electromagnetic radiation emitted by the plasma can be detected to provide an optical-spectrum signal of the sample. A preparation subsystem can add the recognition construct to the sample and a washing subsystem can wash unbound recognition construct out of the sample.Type: GrantFiled: February 12, 2016Date of Patent: December 24, 2019Assignee: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Patent number: 10451556Abstract: An apparatus and method for characterizing a target, e.g., microbial samples or biological toxins, includes labeling the target with a biomolecular recognition construct and measuring an atomic-spectra signal of the biomolecular recognition construct. The method can include heating the labeled target before measuring the atomic-spectra signal. The atomic-spectra signal can be measured by performing laser-induced breakdown spectroscopy. The atomic-spectra signal can be measured by performing spark induced breakdown spectroscopy. The biomolecular recognition construct can be prepared by tagging a biological scaffolding with a metal atom or ion. In an aspect in which the target includes a microbial sample, the biological scaffolding can include an antibody against epitopes present on bacterial surface, the antibody linked to a heavy metal. In an aspect in which the target includes a biological toxin, the biological scaffolding can include an antibody against the biological toxin linked to heavy metals.Type: GrantFiled: September 14, 2015Date of Patent: October 22, 2019Assignee: Purdue Research FoundationInventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Publication number: 20180285624Abstract: A computer method for correlating depictions of colonies of microorganisms includes receiving an image of a substrate associated with a first time and showing a colony of microorganisms. A second image of the same substrate and associated with a second time shows a candidate colony of microorganisms. A region of the second image that shows the candidate colony of microorganisms is located. The first region of the first image is compared to the second region of the second image. Based on the comparison of the images, the candidate colony of microorganism is determined to be the same colony as the first colony of microorganisms. Systems for moving substrates having colonies of microorganisms and maintaining orientation of the substrates before and after movement are also described.Type: ApplicationFiled: April 21, 2016Publication date: October 4, 2018Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery Patsekin
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Publication number: 20180129864Abstract: A system for the characterization of a colony of microorganisms includes a coherent light source configured to provide coherent light of one or more wavelengths along a common optical path. A holder is configured to operationally arrange a substrate so that the colony of microorganisms on a surface of the substrate is positioned to receive the coherent light. Scattered light is generated from the colony of microorganisms receiving coherent light. A first image capture device is configured to receive the scattered light and generate a scatter image from the microorganism colony irradiated by the coherent light. The system also includes a magnifying lens configured to magnify the colony of microorganisms. A second image capture device is configured to capture a light image of the colony of microorganisms magnified by the magnifying lens. Methods of assigning organisms to categories with like organisms without necessarily identifying the organisms are also described.Type: ApplicationFiled: April 21, 2016Publication date: May 10, 2018Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Kathryn E. Ragheb, Cheryl M. Holdman, Valery P. Patsekin, Euiwon Bae, Jennifer Sturgis
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Publication number: 20170307533Abstract: An apparatus and method for characterizing a target, e.g., microbial samples or biological toxins, includes labeling the target with a biomolecular recognition construct and measuring an atomic-spectra signal of the biomolecular recognition construct. The method can include heating the labeled target before measuring the atomic-spectra signal. The atomic-spectra signal can be measured by performing laser-induced breakdown spectroscopy. The atomic-spectra signal can be measured by performing spark induced breakdown spectroscopy. The biomolecular recognition construct can be prepared by tagging a biological scaffolding with a metal atom or ion. In an aspect in which the target includes a microbial sample, the biological scaffolding can include an antibody against epitopes present on bacterial surface, the antibody linked to a heavy metal. In an aspect in which the target includes a biological toxin, the biological scaffolding can include an antibody against the biological toxin linked to heavy metals.Type: ApplicationFiled: September 14, 2015Publication date: October 26, 2017Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Publication number: 20170219485Abstract: A system for the identification of micro-organisms includes an irradiation unit adapted to sequentially provide coherent electromagnetic radiation of one or more wavelengths along a common optical path. A holder is adapted to retain a substrate having a surface adapted for growth of a micro-organism colony. A beamsplitter is adapted to direct the coherent electromagnetic radiation from the common optical path towards the retained substrate. An imager is arranged opposite the beamsplitter from the retained substrate and is adapted to obtain images of backward-scattered light patterns from the micro-organism colony irradiated by the respective wavelengths of the directed coherent electromagnetic radiation. Some examples provide radiation of multiple wavelengths and include an imager arranged optically downstream of the retained substrate to obtain images of forward-scattered light patterns from the micro-organism colony irradiated by the wavelengths of radiation.Type: ApplicationFiled: October 1, 2015Publication date: August 3, 2017Inventors: Euiwon Bae, Arun K. Bhunia, Edwin Daniel Hirleman, Huisung Kim, Bartlomiej P. Rajwa, Joseph Paul Robinson, Valery Patsekin
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Publication number: 20160161415Abstract: A target within a sample can be characterized using an energy source configured to transform a metal in the sample into a plasma and an optical spectroscopic detector configured to detect electromagnetic radiation emitted by the plasma to provide an optical-spectrum signal. A processor can determine presence of the metal in the sample using the optical-spectrum signal. The target can include a microbe or biological toxin. A recognition construct comprising a metal and a scaffold can be applied to the sample. The scaffold can bind to the target. Energy can be applied to transform at least some of the sample into a plasma. Electromagnetic radiation emitted by the plasma can be detected to provide an optical-spectrum signal of the sample. A preparation subsystem can add the recognition construct to the sample and a washing subsystem can wash unbound recognition construct out of the sample.Type: ApplicationFiled: February 12, 2016Publication date: June 9, 2016Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
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Patent number: 8831889Abstract: A system and method of validating differences between measured values of fluorescence intensities obtained from a fluorescence-based instrument, including: generating calibration histograms for calibration beads run through the instrument; calibrating a distance function configured to measure distances between the histograms by: constructing a metric using the distance function, which includes a bin-to-bin dissimilarity matrix; and populating the dissimilarity matrix to maximize the following conditions: (A) the distance function of two histograms representing two sets of different beads equals the difference of their MESF values; and (B) the distance function of two histograms representing fluorescence of two sets of identical beads is zero; applying the metric to flow cytometry histograms generated for biological samples, to determine distances between the histograms; constructing a statistical test using the metric to determine a statistical significance of the distances; and determining whether the histogType: GrantFiled: March 31, 2009Date of Patent: September 9, 2014Assignee: Purdue Research FoundationInventors: Bartlomiej P. Rajwa, Tytus S. Bernas, Joseph P. Robinson
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Publication number: 20110066385Abstract: A system and method of validating differences between measured values of fluorescence intensities obtained from a fluorescence-based instrument, including: generating calibration histograms for calibration beads run through the instrument; calibrating a distance function configured to measure distances between the histograms by: constructing a metric using the distance function, which includes a bin-to-bin dissimilarity matrix; and populating the dissimilarity matrix to maximize the following conditions: (A) the distance function of two histograms representing two sets of different beads equals the difference of their MESF values; and (B) the distance function of two histograms representing fluorescence of two sets of identical beads is zero; applying the metric to flow cytometry histograms generated for biological samples, to determine distances between the histograms; constructing a statistical test using the metric to determine a statistical significance of the distances; and determining whether the histogType: ApplicationFiled: March 31, 2009Publication date: March 17, 2011Applicant: Purdue Research FoundationInventors: Bartlomiej P. Rajwa, Tytus S. Bernas, Joseph P. Robinson