Patents by Inventor Barton Holmquist

Barton Holmquist has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Polypeptide cleavage process
    Patent number: 8288130
    Abstract: A palladate palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladate palladium promoter dissolved in a high-concentration acidic organic acid solvent.
    Type: Grant
    Filed: June 7, 2010
    Date of Patent: October 16, 2012
    Assignee: Medtronic, Inc.
    Inventors: Jin Seog Seo, Daniel Strydom, Barton Holmquist
  • METHOD FOR UNIVERSAL ENZYMATIC PRODUCTION OF BIOACTIVE PEPTIDES
    Publication number: 20120156719
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Application
    Filed: January 15, 2009
    Publication date: June 21, 2012
    Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
  • Composition for palladium-mediated cleavage of peptides containing CXC, CXH or CHM sequences
    Patent number: 8153762
    Abstract: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
    Type: Grant
    Filed: August 28, 2008
    Date of Patent: April 10, 2012
    Assignee: Medtronic, Inc.
    Inventors: Barton Holmquist, Daniel J. Strydom, Jin Seog Seo
  • Methods and constructs for high yield expression of clostripain
    Patent number: 8003362
    Abstract: The invention provides methods and nucleic acid constructs to express clostripain. The source of the coding region for recombinantly expressed clostripain is Clostridium histolyticum.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: August 23, 2011
    Assignee: Medtronic Inc.
    Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Barton Holmquist
  • POLYPEPTIDE CLEAVAGE PROCESS
    Publication number: 20100311947
    Abstract: A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent.
    Type: Application
    Filed: June 7, 2010
    Publication date: December 9, 2010
    Applicant: MEDTRONIC, INC.
    Inventors: Jin Seog Seo, Daniel Strydom, Barton Holmquist
  • Polypeptide cleavage process
    Patent number: 7771966
    Abstract: A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: August 10, 2010
    Assignee: Medtronic, Inc.
    Inventors: Jin Seog Seo, Daniel Strydom, Barton Holmquist
  • Polypeptide cleavage process
    Patent number: 7592432
    Abstract: The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is between about 1 to about 22 molar.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: September 22, 2009
    Assignee: Restoragen, Inc.
    Inventors: Barton Holmquist, Daniel Strydom, Jin Seog Seo, Kirk Cryer
  • PEPTIDE AMIDATION PROCESS
    Publication number: 20090215987
    Abstract: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
    Type: Application
    Filed: August 28, 2008
    Publication date: August 27, 2009
    Inventors: Barton Holmquist, Daniel Strydom
  • Method for universal enzymatic production of bioactive peptides
    Patent number: 7498148
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: March 3, 2009
    Assignee: Restoragen, Inc.
    Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
  • Peptide amidation process
    Patent number: 7462690
    Abstract: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: December 9, 2008
    Assignee: Restoragen, Inc.
    Inventors: Barton Holmquist, Daniel Strydom
  • METHOD FOR UNIVERSAL ENZYMATIC PRODUCTION OF BIOACTIVE PEPTIDES
    Publication number: 20080153129
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Application
    Filed: November 21, 2007
    Publication date: June 26, 2008
    Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
  • Method for universal enzymatic production of bioactive peptides
    Patent number: 7335486
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: February 26, 2008
    Assignee: Restoragen, Inc.
    Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
  • PEPTIDE PHARMACEUTICAL FORMULATIONS
    Publication number: 20060183685
    Abstract: A pharmaceutical composition for administration to a mammal is disclosed. The composition includes a therapeutically effective amount of a peptide, such as a GLP-1 molecule, a PTH molecule, or a GRF molecule. The composition further includes a buffer including a weak acid having an acid dissociation constant value of greater than about 1×10?5, such as acetic acid. The composition also includes an excipient for making the composition generally isotonic, such as D-mannitol.
    Type: Application
    Filed: April 24, 2006
    Publication date: August 17, 2006
    Inventors: Barton Holmquist, Daniel Dormady
  • Method for universal enzymatic production of bioactive peptides
    Publication number: 20060008870
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Application
    Filed: November 24, 2004
    Publication date: January 12, 2006
    Inventors: Fred Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin Merrifield, Mary Bossard, Barton Holmquist, Jin Seo
  • Peptide amidation process
    Publication number: 20050287632
    Abstract: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
    Type: Application
    Filed: November 24, 2004
    Publication date: December 29, 2005
    Inventors: Barton Holmquist, Daniel Strydom
  • Method for enzymatic production of GLP-1 (7-36) amide peptides
    Publication number: 20050260701
    Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
    Type: Application
    Filed: November 24, 2004
    Publication date: November 24, 2005
    Inventors: Fred Wagner, Peng Luan, Yuannan Xia, Mary Bossard, Barton Holmquist, Edwin Merrifield, Daniel Strydom
  • Methods and constructs for high yield expression of clostripain
    Publication number: 20050244924
    Abstract: The invention provides methods and nucleic acid constructs to express clostripain.
    Type: Application
    Filed: November 24, 2004
    Publication date: November 3, 2005
    Inventors: Fred Wagner, Peng Luan, Yuannan Xia, Barton Holmquist
  • Methods and DNA constructs for high yield production of polypeptides
    Publication number: 20050239172
    Abstract: The invention provides an inclusion body fusion partner to increase peptide and polypeptide production in a cell.
    Type: Application
    Filed: November 24, 2004
    Publication date: October 27, 2005
    Inventors: Fred Wagner, Peng Luan, Yuannan Xia, Barton Holmquist
  • Polypeptide cleavage process
    Publication number: 20050227313
    Abstract: A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent.
    Type: Application
    Filed: November 24, 2004
    Publication date: October 13, 2005
    Inventors: Jin Seo, Daniel Strydom, Barton Holmquist
  • Polypeptide cleavage process
    Publication number: 20050227314
    Abstract: The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is between about 1 to about 22 molar.
    Type: Application
    Filed: November 24, 2004
    Publication date: October 13, 2005
    Inventors: Barton Holmquist, Daniel Strydom, Jin Seo, Kirk Cryer