Abstract: The present invention relates in general to the field of rapid detection and quantification of tissue/organ injury, and more particularly, to a novel platform for monitoring and quantification of circulating cell-free DNA including within extracellular vesicles, together with extracellular vesicles specific markers, and extracellular vesicles sizes also in combination with PCR-related technologies in biospecimens, and liquid biopsies for the assessment and prediction of severity of tissue/organ injury, monitoring of disease progression as well as the assessment of the response to therapeutic interventions.