Abstract: A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ?- or ?-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ?- or ?-1 functionalized acyl-CoAs as a substrate; a method of producing a ?-1 hydroxy branched fatty acid, a ?-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ?-1 hydroxy branched fatty acids, a ?-1 branched fatty acids, or a combination thereof obtained from the culture.

Abstract: A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ?- or ?-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ?- or ?-1 functionalized acyl-CoAs as a substrate; a method of producing a ?-1 hydroxy branched fatty acid, a ?-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ?-1 hydroxy branched fatty acids, a ?-1 branched fatty acids, or a combination thereof obtained from the culture.

Abstract: The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant.