Abstract: A TROP2 targeting antibody-drug conjugate, and a preparation method and use therefor. The antibody-drug conjugate is shown in formula I. Said type of compound has a good targeting property, a good inhibitory effect on tumor cells positively expressing TROP2, and good druggability and high safety. The present antibody drug conjugate has an inhibitory effect on TROP2, and also a good inhibitory effect on at least one of NCI-N87, MDA-MB-468 and BXPC3 cells.

Abstract: An automatic detection function-integrated detection apparatus includes a drill rod and an elastic water storage bag; two sides of the drill rod are a head region and a tail region, respectively; an interior of the drill rod has a water supply channel, a side wall of the drill rod is provided with a first channel, and a first valve is disposed in the first channel. The automatic detection function-integrated detection apparatus and method for the water flowing fractured zone may complete the detection of the water flowing fractured zone while drilling, and thus the labor intensity of workers may be reduced.

Abstract: Provided are base editors containing a cytidine and a catalytically inactive version of Lachnospiraceae bacterium Cpf1 (LbCpf1). The new base editors have greatly improved editing efficiency and fidelity as compared to Cas9-based base editors, and have different editing windows.

Abstract: Provided are gene therapy technologies, including specifically designed and tested guide RNA sequences for improved base editors, useful for increasing the expression of the gamma-globin gene. The guide RNA sequences may target the BCL11A erythroid enhancer or the gamma-globin promoter, or both at the same time. The base editors can include nucleobase deaminase inhibitor that inhibits the editing activity of the base editors until they are bound to the target sites. These gene therapy technologies are useful for treating diseases including beta-thalassemia and sickle cell anemia, among others.

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

Abstract: Provided are compositions and methods for enhanced prime editing, which include a pegRNA that encodes a target mutation in a target protein, along with one or more nearby silent or conservative mutations. These silent mutations can increase the editing efficiency, without causing a change to the target protein sequence. Also provided are compositions and methods of using the improved prime editing for preventing or treating infections by SARS-CoV or SARS-CoV-2.

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

Abstract: In an approach to predicting issue development trends based on generated ordered association rules, one or more computer processors subdivide an issue into a set of one or more subproblems. The one or more computer processors generate ordered association rules by inputting the set of one or more subproblems into a model trained with historical subproblems, historical solutions, and historical ordered association rules. The one or more computer processors determine one or more solutions for each subproblem in the set of one or more subproblems utilizing the generated ordered association rules. The one or more computer processors present the one or more determined solutions.

Abstract: Provided are base editors containing a cytidine and a catalytically inactive version of Lachnospiraceae bacterium Cpf1 (LbCpf1). The new base editors have greatly improved editing efficiency and fidelity as compared to Cas9-based base editors, and have different editing windows.

Abstract: An information processing method, which is applied to a server, includes: a public message is transmitted to a first client, the public message being a message issued to the first client by the server, the public message carrying first information and a second information set including N pieces of second information, with N being a positive integer, the first information is information only allowed to be gotten by the first client and the second information is information only allowed to be gotten by a client in a sharing relationship with the first client; and a request for getting the second information is received from a second client, it is verified whether the second client is in the sharing relationship with the first client, and after the second client is determined to be in the sharing relationship with the first client, the second client is allowed to get the second information.

Abstract: The present application relates generally to a projection system and method for projecting content onto a moving performer. In one embodiment, the system includes a projector that projects light onto the performer, a position sensor, such as a camera and/or depth sensor that captures position information about the performer, and a computer that modifies original content based on the position information of the performer. The computer uses the position information to generate a mask, such as a silhouette, of the performer, and then modifies the original content based on the mask of the performer. The modified content is then projected by the projector onto the performer.

Abstract: Embodiments of the present disclosure provide a temperature compensation circuit, a display panel and a temperature compensation method. The temperature compensation circuit comprises: a temperature sensing unit, adapted to sense a temperature of an external environment; a temperature compensation control unit being adapted to compare the temperature sensed output voltage with a reference voltage, and generate a control signal based on the comparison result; and a first voltage source adapted to receive a control signal from the temperature compensation control unit, generate a corresponding driving voltage based on the control signal and output the corresponding driving voltage to a gate drive circuit as a gate driving voltage of the gate drive circuit, and generate a feedback signal according to the control signal and output the feedback signal to the temperature sensing unit and the temperature compensation control unit, the reference voltage being variable based on the feedback signal.

Abstract: Embodiments of the present disclosure provide a temperature compensation circuit, a display panel and a temperature compensation method. The temperature compensation circuit comprises: a temperature sensing unit, adapted to sense a temperature of an external environment; a temperature compensation control unit being adapted to compare the temperature sensed output voltage with a reference voltage, and generate a control signal based on the comparison result; and a first voltage source adapted to receive a control signal from the temperature compensation control unit, generate a corresponding driving voltage based on the control signal and output the corresponding driving voltage to a gate drive circuit as a gate driving voltage of the gate drive circuit, and generate a feedback signal according to the control signal and output the feedback signal to the temperature sensing unit and the temperature compensation control unit, the reference voltage being variable based on the feedback signal.

Abstract: An information processing method, which is applied to a server, includes: a public message is transmitted to a first client, the public message being a message issued to the first client by the server, the public message carrying first information and a second information set including N pieces of second information, with N being a positive integer, the first information is information only allowed to be gotten by the first client and the second information is information only allowed to be gotten by a client in a sharing relationship with the first client; and a request for getting the second information is received from a second client, it is verified whether the second client is in the sharing relationship with the first client, and after the second client is determined to be in the sharing relationship with the first client, the second client is allowed to get the second information.