Abstract: The present invention, belonging to the technical field of biological imaging, discloses a photo-activatable aggregation-induced emission probe with in-situ generation capability, a preparation method therefor and an application thereof. The preparation method is as follows: A bis(2-(2-hydroxybenzylidene)amino)aryl disulfide compound is reacted with thiol to obtain a photo-activatable aggregation-induced emission probe, which has a structure as shown in formula III. In the present invention, the photo-activatable aggregation-induced emission probe is aggregated into a specific organelle, and a 2-(2-hydroxyphenyl)benzothiazole compound having the aggregation-induced emission property is generated through a photo-oxidation reaction.

Abstract: The present application provides a catalyst for copolymerization of ethylene and methyl methacrylate and an application thereof. The catalyst includes a main catalyst, and the main catalyst is obtained by compounding a compound of formula (I) and a compound of formula (II); a mole ratio of the compound of formula (I) to the compound of formula (II) is (1:49) to (49:1). The catalyst has an excellent catalytic activity, and a copolymer of ethylene and methyl methacrylate with a high molecular weight can be obtained, with a weight average molecular weight of not less than 1×105 g/mol.

Abstract: This invention is to provide a method for detecting DNA hybridization on DNA chips using 4-D parameters. Based on the traditional method of using 3-D parameters to detect DNA hybridization on DNA chips, this invention introduces temperature as the forth parameters to determine the melting temperatures by scanning the changes of fluorescence intensity of the fluorescent labels on the double-stranded oligo nuclei acids resulted from the increasing of hybridization temperature. Comparing the measured melting temperatures with the standard melting temperature specific for each probe on the chip is used to gain insight into the specific property of the single-stranded nucleic acids in the sample. This device is composed of a DNA chip containing transparent glass chamber, in which a temperature sensor and a thermo-circler are installed. This invention provides a specific, sensitive, and easy operating method to detect gene in a complex sample.

Abstract: This invention is to provide a method for detecting DNA hybridization on DNA chips using 4-D parameters. Based on the traditional method of using 3-D parameters to detect DNA hybridization on DNA chips, this invention introduces temperature as the forth parameters to determine the melting temperatures by scanning the changes of fluorescence intensity of the fluorescent labels on the double-stranded oligo nuclei acids resulted from the increasing of hybridization temperature. Comparing the measured melting temperatures with the standard melting temperature specific for each probe on the chip is used to gain insight into the specific property of the single-stranded nucleic acids in the sample. This device is composed of a DNA chip containing transparent glass chamber, in which a temperature sensor and a thermo-circler are installed. This invention provides a specific, sensitive, and easy operating method to detect gene in a complex sample.