Abstract: The present disclosure relates to methods for generating blood protein profiles in red blood cell-enriched blood samples. The disclosed methods represent a new and improved laboratory technique for producing a protein profile from blood, increasing protein detection.

Abstract: The present disclosure relates to methods for modulating the level of proteins in a subject or in target cells by priming red blood cells with various agents or conditions that modulate the levels of proteins associated with red blood cells and administering the primed red blood cells to a subject. The disclosed methods represent a novel use of red blood cells primed to express a number of proteins, as cell therapies for numerous diseases or disorders.

Abstract: The present disclosure relates to methods for generating blood protein profiles in red blood cell-enriched blood samples. The disclosed methods represent a new and improved laboratory technique for producing a protein profile from blood, increasing protein detection.

Abstract: The invention relates to methods for monitoring progression of an inflammatory condition in a subject. In particular embodiments the patient is undergoing treatment of the inflammatory condition and the method comprises monitoring the level of one or more biomarkers to monitor disease progression, for example to assist the clinician in optimizing the treatment regimen. In particular embodiments the subject is undergoing treatment with mesenchymal stem cells (MSCs). In particular embodiments the invention relates to methods of monitoring the effectiveness of autologous or allogeneic cell therapy of a patient having a condition characterized by cartilage damage or degeneration, such as OA, for example in order to assist a practitioner in determining an appropriate time to administer a further dose of cells. The invention also provides kits and components for use in the methods.

Abstract: The present disclosure relates to methods for modulating the level of proteins in a subject or in target cells by priming red blood cells with various agents or conditions that modulate the levels of proteins associated with red blood cells and administering the primed red blood cells to a subject. The disclosed methods represent a novel use of red blood cells primed to express a number of proteins, as cell therapies for numerous diseases or disorders.

Abstract: The present disclosure relates to methods for generating blood protein profiles in red blood cell-enriched blood samples. The disclosed methods represent a new and improved laboratory technique for producing a protein profile from blood, increasing protein detection.

Abstract: The invention relates to methods for monitoring progression of an inflammatory condition in a subject In particular embodiments the patient is undergoing treatment of the inflammatory condition and the method comprises monitoring the level of one or more biomarkers to monitor disease progression, for example to assist the clinician in optimising the treatment regimen. In particular embodiments the subject is undergoing treatment with mesenchymal stem cells (MSCs). In particular embodiments the invention relates to methods of monitoring the effectiveness of autologous or allogeneic cell therapy of a patient having a condition characterised by cartilage damage or degeneration, such as OA, for example in order to assist a practitioner in determining an appropriate time to administer a further dose of cells. The invention also provides kits and components for use in the methods.

Abstract: An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

Abstract: An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

Abstract: An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

Abstract: An apparatus for rehydrating and for performing electrophoresis on a gel strip includes a tray defining a plurality of parallel troughs configured to receive gel strips. Each trough defines a centrally located rehydration area and an electrode area disposed either side of the electrode area. End walls delimit the rehydration area of the trough from the electrode area. Electrode means including contact points adapted to contact either the gel strip in the electrode areas near the first and second end of the gel or a conducting or current carrying electrode bridge material which is in contact with the gel strip in the electrode areas. The electrode means are adapted to be connected to a means for supplying an electric current for imposing an electric potential in the strip between the electrodes.

Abstract: A method for separating a sample into components by two-dimensional electrophoresis uses an IPG strip, and a gel slab which are spaced apart and carried on a single generally planar support means. The planar support means is first oriented in a generally vertical plane and the first electrophoretic separation medium is oriented in a horizontal plane spaced above or below the second electrophoretic separation medium by a gap. A first dimension separation of a sample mixture in the strip is then carried out while the strip and slab are separated by a non-electrically conducting liquid which is substantially immiscible with water and is non-extractive of water preferably paraffin oil. After the first separation has been carried the support means is tilted so that the first strip is at an angle to the horizontal and the paraffin is flushed out from the gap between the strip and the slab.

Abstract: A method of screening or detecting non-ocular disease in an animal, the method comprising the steps of: (a) obtaining a tear sample from the animal; (b) separating biomolecules present in the tear sample; and (c) detecting for the presence or absence of one or more biomolecules such that the presence or absence of the one or more biomolecules being an indicator or marker of a disease state in the animal.

Abstract: A method for separating a sample into components by two-dimensional electrophoresis uses an IPG strip, and a gel slab which are spaced apart and carried on a single generally planar support means. The planar support means is first oriented in a generally vertical plane and the first electrophoretic separation medium is oriented in a horizontal plane spaced above or below the second electrophoretic separation medium by a gap. A first dimension separation of a sample mixture in the strip is then carried out while the strip and slab are separated by a non-electrically conducting liquid which is substantially immiscible with water and is non-extractive of water preferably paraffin oil. After the first separation has been carried the support means is tilted so that the first strip is at an angle to the horizontal and the paraffin is flushed out from the gap between the strip and the slab.

Abstract: The present invention is directed to an immobilised pH gradient (IPG) gel for use in electrophoresis, the gel comprising polymerised units of (I) CH2═CR1—CO—NR2R3 and (II) CH2═CR4—CO—NR5R6 wherein R1, R2, R3, R4, R5, and R6, are the same or different and are hydrogen or C1-C4alkyl, with the proviso that at least one of R1, R2, R3, R4, R5, or R6 is C1-C4alkyl.

Abstract: An immobilised pH gradient (IPG) gel comprising a polymerised mixture of monomers comprising: (I) CH2═CR1—CO—NR2R3, (II) (CH2═CHCONHCH2CH2S—)2 (BAC) and optionally (III) a non-reducible crosslinker, wherein R1, R2 and R3 are the same or different and are hydrogen or optionally substituted alkyl or cycloakyl.

Abstract: An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

Abstract: An electrophoresis apparatus for the simultaneous electrophoretic separation of a plurality of component mixtures using a disposable tray having a plurality of parallel troughs shaped to retain an elongate strip of isoelectric focusing medium and sufficient liquid to immerse the elongate strip. A frame is provided to retain the tray in a horizontal position using a pair of rotatably mounted support members moving between an open and closed position. Support members are also connected to a plurality of electrodes aligned with each of the troughs so that the electrodes are positioned clear of the troughs in the open position and positioned in the troughs when in the closed position.

Abstract: A method for separating a sample into components by two-dimensional electrophoresis uses an IPG strip, and a gel slab which are spaced apart and carried on a single generally planar support means. The planar support means is first oriented in a generally vertical plane and the first electrophoretic separation medium is oriented in a horizontal plane spaced above or below the second electrophoretic separation medium by a gap. A first dimension separation of a sample mixture in the strip is then carried out while the strip and slab are separated by a non-electrically conducting liquid which is substantially immiscible with water and is non-extractive of water preferably paraffin oil. After the first separation has been carried the support means is tilted so that the first strip is at an angle to the horizontal and the paraffin is flushed out from the gap between the strip and the slab.