Abstract: Systems for treating water are disclosed. The system includes an ozonation subsystem including a source of ozone configured to dissolve ozone into water from a source of water to produce ozonated water. The system further includes an electrochemical cell co-located with the ozonation subsystem having an inlet connectable to a source of electrolyte and an outlet. The electrochemical cell is configured to produce hydrogen peroxide from electrolyte from the source of electrolyte. The system additionally includes a mixing zone configured to receive the ozonated water, to receive the hydrogen peroxide from the outlet of the electrochemical cell, and to mix the ozonated water and hydrogen peroxide to form peroxone. Systems and methods of treating water, such as by selectively removing one or more emergent compounds from contaminated water, using the system are also disclosed.

Abstract: Provided herein is a method for detecting the presence or absence of at least one of Clostridium botulinum toxin gene A, B, E, and F in a biological sample by means of PCR amplification using toxin specific primers and labeled probes in connection with real time or delayed detection. Also provided are specific primer and probe sequences, a diagnostic method and a kit comprising primers and probes for detection of toxin genes A, B, E, or F in a biological sample.

Abstract: Provided herein is a method for detecting the presence or absence of at least one of Clostridium botulinum toxin gene A, B, E, and F in a biological sample by means of PCR amplification using toxin specific primers and labeled probes in connection with real time or delayed detection. Also provided are specific primer and probe sequences, a diagnostic method and a kit comprising primers and probes for detection of toxin genes A, B, E, or F in a biological sample.