Abstract: Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.

Abstract: Disclosed herein are methods to maintain or inhibit an alteration in volume or shape of hydrated decellularized extracellular matrix from a mammalian organ or tissue or a portion thereof.

Abstract: Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.

Abstract: A method of extracellular matrix (ECM) particle formation, and compositions produced by the method, are provided.

Abstract: Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.

Abstract: Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.