Abstract: Method for the disinfection and the quality control of the disinfection of the hands of a user comprising the steps of:—dispensing a predetermined amount of the mixture of a disinfectant substance and a light reflection modifying substance on the hands to be disinfected,—allowing the user to smear and rub the mixture on his/her hands,—inserting the hands one after the other into an imaging compartment (11) of an apparatus (10) for disinfection quality control;—illuminating the inserted hand with light sources (14) emitting light having a predetermined spectral distribution and intensity;—recording the image of the hand (9) from both sides by cameras (16);—processing the recorded image to provide distinction between areas of the hand contacted and non-contacted by the mixture, wherein the light reflection modifying substance is a substance that absorbs light in at least a predetermined wavelength range which is added to the disinfectant substance in an amount that decreases light absorption of areas treated by