Abstract: The invention relates to a method of separating immunoglobulin variants, comprising the steps of: a) providing a column packed with an Fc-binding affinity chromatography resin; b) loading a sample comprising at least two Fc-comprising immunoglobulin variants onto the column; c) optionally washing the column with a washing liquid; and d) conveying an eluent through said column to elute at least a target immunoglobulin variant from said column and recovering one or more eluate fractions comprising the target immunoglobulin variant in enriched form.

Abstract: A tool for fastening an elongated object on a supporting surface with U-shaped clips, each embracing the elongated object and being secured to the supporting surface by a fastening member extending through one of its legs. The tool comprises a magazine for receiving a succession of clips to be successively fed through the magazine, a feed device which is adapted to feed the succession of clips in a direction towards a determined mounting position at the front end of the tool, and a driving device with a driver for driving the fastening member of a clip in the mounting position into the supporting surface. The magazine is designed to receive the clips with their fastening members in such an orientation that each clip has its U-legs disposed after each other in the longitudinal direction of the magazine so that it may be secured over the elongated object oriented transversely of the longitudinal direction of the magazine.