Patents by Inventor Bengt Bjellqvist
Bengt Bjellqvist has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9341595Abstract: An electrophoresis gel assembly comprising a gel slab, at least two sample wells and at least one focusing buffer well arranged between two adjacent sample wells and wherein focusing barriers are arranged between the focusing buffer well and the sample wells. A high conductivity focusing buffer can be applied to the focusing buffer well to obtain a lateral focusing of the sample bands during electrophoresis.Type: GrantFiled: January 10, 2011Date of Patent: May 17, 2016Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Elsemarie Bjellqvist, Henrik Ostlin
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Patent number: 8858771Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilized pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.Type: GrantFiled: February 10, 2012Date of Patent: October 14, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Kristina Uhlen
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Publication number: 20120228121Abstract: The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.Type: ApplicationFiled: November 26, 2010Publication date: September 13, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Erik Bjerneld, Jan-Olof Johansson, Ronnie Palmgren
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Publication number: 20120138464Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.Type: ApplicationFiled: February 10, 2012Publication date: June 7, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: BENGT BJELLQVIST, KRISTINA UHLÉN
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Patent number: 8187438Abstract: This invention relates to electrophoresis, in particular pre-cast mini gels with a low fluorescent polymer backing that is not adherent to the gel. Use of this type of small gel simplifies blotting procedures, such as Southern and Western blotting, after electrophoresis.Type: GrantFiled: September 24, 2007Date of Patent: May 29, 2012Assignee: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Sofia Edlund, Jesper Hedberg
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Patent number: 7989215Abstract: The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.Type: GrantFiled: June 9, 2006Date of Patent: August 2, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Jonas Astrom, Bengt Bjellqvist, Lars Fagerstam, Kristina Uhlen
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Patent number: 7964075Abstract: The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilized pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.Type: GrantFiled: April 20, 2007Date of Patent: June 21, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Kristina Uhlen
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Patent number: 7943029Abstract: The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.Type: GrantFiled: January 30, 2007Date of Patent: May 17, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Henrik Neu, Kristina Uhlen
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Publication number: 20100243453Abstract: This invention relates to electrophoresis, in particular pre-cast mini gels with a low fluorescent polymer backing that is not adherent to the gel. Use of this type of small gel simplifies blotting procedures, such as Southern and Western blotting, after electrophoresis.Type: ApplicationFiled: September 24, 2007Publication date: September 30, 2010Applicant: GE Healthcare Bio-Science ABInventors: Bengt Bjellqvist, Sofia Edlund, Jesper Hedberg
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Publication number: 20100213065Abstract: The present invention relates to methods and devices for forming a plurality of wells on a gel containing an analyte. The invention further provides a system for eluting a liquid analyte reagent mixture from a gel. The invention is useful in the separation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for separating and eluting peptides from isoelectric focusing gels.Type: ApplicationFiled: June 9, 2006Publication date: August 26, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jonas Astrom, Bengt Bjellqvist
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Publication number: 20100181197Abstract: The present invention relates to the field of isoelectric focussing or 2D electrophoresis, in particular isoelectric point markers with fluorescence detection. The marker comprises an oligopeptide covalently labelled with a fluorescent dye and is characterised in that the dye has a net charge that will maintain the overall net charge of the oligopeptide upon being bound to the oligopeptide. The invention also relates to an electrophoretic method for analysis of proteins using these markers.Type: ApplicationFiled: July 14, 2008Publication date: July 22, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Miles W. Burrrows
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Publication number: 20100163416Abstract: The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilised pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.Type: ApplicationFiled: April 20, 2007Publication date: July 1, 2010Applicant: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Kristina Uhlen
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Publication number: 20100143895Abstract: The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.Type: ApplicationFiled: June 9, 2006Publication date: June 10, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jonas Astrom, Bengt Bjellqvist, Lars Fagerstam, Kristina Uhlen
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Publication number: 20090026081Abstract: The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.Type: ApplicationFiled: January 30, 2007Publication date: January 29, 2009Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Henrik Neu, Kristina Uhlen
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Publication number: 20080318327Abstract: The present invention relates to a kit for mass labelling of peptides in two or more samples, comprising a set of mass tagging reagents for reaction at the N-terminals of the peptides and a set of mass balancing reagents for reaction at the C-terminals of the peptides, or vice versa, and wherein each mass tagging reagent in the set is matched with mass balancing reagent(s) in such a way that the sum of the masses from all matched mass tagging and mass balancing reagents is equal. The invention also relates to a method of using said mass labels and a database for use in said method.Type: ApplicationFiled: December 11, 2006Publication date: December 25, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, David Fenyo, Henrik Neu, Jesper Hedberg
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Publication number: 20080293083Abstract: The present invention relates to a method for peptide analysis, comprising the following steps: a) tagging N-terminals of peptides in sample(s) with mass tagging reagent(s) and mass balancing C-terminals of said peptides with mass balancing reagent(s), or vice versa; and b) mass spectrometry analysis of said peptides. The present invention also relates to a kit with global mass tagging reagents and mass balancing reagents for use in said method and a database with specific peptide information.Type: ApplicationFiled: July 5, 2005Publication date: November 27, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, David Fenyo, Jesper Hedberg, Henrik Neu
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Publication number: 20080289963Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.Type: ApplicationFiled: December 19, 2006Publication date: November 27, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Kristina Uhlen
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Publication number: 20070187249Abstract: The present invention relates to a novel use of a positively charged support, namely as a sample loading support for loading samples onto an IPG (Immobilised pH gradient) gel. The support or paper is provided with positively charged groups, such as cation groups, and is used to load samples from the cathode side of the IPG gel. Furthermore, the invention relates to a kit comprising a positively charged sample application support as above and an IPG gel, preferably a pre-swollen RTG gel comprising an acidic pH-interval.Type: ApplicationFiled: December 15, 2004Publication date: August 16, 2007Inventor: Bengt Bjellqvist
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Patent number: 7147762Abstract: The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilized medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide-comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises reactive disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium.Type: GrantFiled: August 29, 2002Date of Patent: December 12, 2006Assignee: GE Healthcare Bio-Sciences ABInventors: Bengt Bjellqvist, Ingmar Olsson, Ronnie Palmgren
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Patent number: 7060776Abstract: The invention relates to an acrylic monomer defined by formula (I): wherein R1, R2 and R3 are H or CH3; R4 and R5 are H or OH; R6 and R7 are H or OH; R8 is H or CH3; m is 2–4; and n and p are 0 or 1. The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.Type: GrantFiled: December 17, 2002Date of Patent: June 13, 2006Assignee: GE Healthcare Bio-Sciences ABInventors: Mattias Algotsson, Bengt Bjellqvist, Ronnie Palmgren