Patents by Inventor Benjamin A. Bowen

Benjamin A. Bowen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9222098
    Abstract: Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site.
    Type: Grant
    Filed: August 12, 2003
    Date of Patent: December 29, 2015
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 8735158
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: February 28, 2008
    Date of Patent: May 27, 2014
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 8536420
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: September 17, 2013
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 8143504
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: October 22, 2010
    Date of Patent: March 27, 2012
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Publication number: 20110030107
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: October 22, 2010
    Publication date: February 3, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7820880
    Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
    Type: Grant
    Filed: November 6, 2008
    Date of Patent: October 26, 2010
    Assignee: Pioneer Hi-Bred Int'l. Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7692061
    Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: April 6, 2010
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Kanwarpal S Dhugga, Timothy G Helentjaris, Benjamin A Bowen, Xun Wang
  • Patent number: 7582808
    Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
    Type: Grant
    Filed: October 4, 2007
    Date of Patent: September 1, 2009
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Kanwarpal S Dhugga, Timothy G Helentjaris, Benjamin A Bowen, Xun Wang
  • Patent number: 7572634
    Abstract: Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites. Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene. By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.
    Type: Grant
    Filed: May 7, 2003
    Date of Patent: August 11, 2009
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Publication number: 20090151017
    Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
    Type: Application
    Filed: October 30, 2008
    Publication date: June 11, 2009
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Kanwarpal S. Dhugga, Timothy G. Helentjaris, Benjamin A. Bowen, Xun Wang
  • Publication number: 20090093059
    Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
    Type: Application
    Filed: November 6, 2008
    Publication date: April 9, 2009
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Publication number: 20080320617
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: August 1, 2008
    Publication date: December 25, 2008
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7462766
    Abstract: Compositions comprising non-identical recombination sites are provided. Compositions include plants, plant parts and plant cells comprising transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites.
    Type: Grant
    Filed: May 4, 2006
    Date of Patent: December 9, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Publication number: 20080209595
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: February 28, 2008
    Publication date: August 28, 2008
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7405079
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: May 7, 2003
    Date of Patent: July 29, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7361508
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: May 16, 2003
    Date of Patent: April 22, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7214852
    Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
    Type: Grant
    Filed: October 8, 2004
    Date of Patent: May 8, 2007
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Kanwarpal S. Dhugga, Timothy G. Helentjaris, Benjamin A. Bowen, Xun Wang
  • Patent number: 7148406
    Abstract: The present invention provides methods and compositions relating to altering lignin biosynthesis content and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins which are involved in lignin biosynthesis. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
    Type: Grant
    Filed: February 10, 2003
    Date of Patent: December 12, 2006
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Timothy G. Helentjaris, Benjamin A. Bowen, Xun Wang
  • Patent number: 7102055
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: December 6, 1999
    Date of Patent: September 5, 2006
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 6911575
    Abstract: Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
    Type: Grant
    Filed: May 26, 2000
    Date of Patent: June 28, 2005
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, John H. Duesing, David J. Peterson, Laura A. Tagliani, Tong Zhu