Abstract: Provided is an rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid molecules comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid molecules.

Abstract: The inventors surprisingly found that specific oligosaccharides are capable of inhibiting viral replication through inhibiting NF-?B activation. The invention thus pertains to a composition comprising pectin (in the form of digalacturonic acid, trigalacturonic acid, polygalacturonic acid), Arabinoxylan from rice bran, ?-glucan from bakers yeast, D-Ribose or mixtures there-of for inhibiting viral replication in a mammal with a viral disease.

Abstract: The invention provides improved nucleic acids for anti-HIV therapy. The invention further provides selection methods which are capable of predicting already at an early stage of development whether promising anti-pathogenic candidate compounds will be suitable for therapeutic use in vivo.

Abstract: The invention provides a method for producing a stabilized cell of interest, said method comprising providing a stem cell and/or a precursor cell of said cell of interest with a nucleic acid sequence which, when present in said cell of interest, is capable of stabilizing said cell of interest, providing a non-human animal with said stem cell and/or precursor cell, allowing generation of said cell of interest in said animal, and obtaining said cell of interest. Said animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.

Abstract: The invention provides improved rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid sequences comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid sequences.

Abstract: The invention provides a nucleic acid molecule comprising a sequence that is complementary to a mutant of a genomic nucleic acid sequence of an organism capable of mutating its genome. The genomic nucleic acid sequence preferably comprises a conserved, essential region. Furthermore, the genomic nucleic acid sequence preferably comprises a coding sequence and a DNA/RNA regulatory sequence. A pharmaceutical composition and a gene delivery vehicle comprising a nucleic acid molecule of the invention is also provided, as well as a method for counteracting an organism comprising providing the organism with the nucleic acid molecule. The organism is preferably additionally provided with a nucleic acid sequence complementary to a genomic nucleic acid sequence of the organism.

Abstract: The invention provides a method for producing a stabilized cell of interest, the method comprising providing a stem cell and/or a precursor cell of the cell of interest with a nucleic acid sequence which, when present in the cell of interest, is capable of stabilizing the cell of interest, providing a non-human animal with the stem cell and/or precursor cell, allowing generation of the cell of interest in said animal, and obtaining said cell of interest. The animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.

Abstract: An inducible viral replicon comprising at least one inducible repressor and/or activator, and all viral sequences which are essential for replication under direct or indirect control of said inducible repressor and/or activator. The inducible repressor and/or activator may comprise a Tet operon. A replicon of the invention may be used to produce a virus, for instance an attenuated HIV virus, which can be induced to replicate by the presence of doxycycline or a functional analog thereof. The replicon and/or the produced virus may be used to prepare a vaccine, for instance for the prophylaxis of AIDS. A replicon of the invention can be modified, preferably improved, by culturing the replicon in permissive cells for an extended period of time.

Abstract: Provided is an rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid molecules comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid molecules.