Abstract: Methods are disclosed herein for treating glaucoma in a subject. In some embodiments, the methods increase retinal ganglion cell survival. The disclosed methods use exosomes and/or miRNA.

Abstract: There is disclosed a modular anaerobic digestion point-of-waste to renewable energy system. The system is directed to a modular and scalable anaerobic digestion system for point-of-waste use. The System includes a pretreatment process for removing inhibitory nutrients from a feedstock, an in-treatment process for providing clean renewable energy and a post-treatment process for further providing clean renewable energy for subsequent use. The System includes a leaching bed; a liquids tank; a mixing tank; an anaerobic digester reactor; a precipitation tank; a stripping tank; a hydrogen sulfide scrubber; a water remover; a gas bladder; a dewaterer; and a flare system.

Abstract: Aircraft, vibration isolation assemblies, and methods of assembling vibration isolation assemblies are provided. An aircraft includes a vibration isolation assembly. The vibration isolation assembly includes a mounting track, a plate, a clamp block, a vibration isolator, and a support fitting. The mounting track defines a cavity and includes flanges that define an opening to the cavity. The plate is configured to be positioned on the flanges outside of the cavity. The clamp block is configured to be fastened to the plate under the flanges of the mounting track within the cavity. The vibration isolator is configured to be laterally constrained by the clamp block within the cavity. The support fitting is configured to be secured to the vibration isolator outside of the cavity.

Abstract: A method for determining a desired intake manifold pressure of an internal combustion engine by means of an iterative method, wherein a cylinder charge is determined for an intake manifold pressure iterated during the iterative method, and the desired intake manifold pressure is determined as a function of the cylinder air charge that has been determined. In addition, a control device for carrying out the method is provided.

Abstract: Methods are disclosed herein for treating glaucoma in a subject. In some embodiments, the methods increase retinal ganglion cell survival. The disclosed methods use exosomes and/or miRNA.

Abstract: Methods are disclosed herein for treating glaucoma in a subject. In some embodiments, the methods increase retinal ganglion cell survival. The disclosed method use exosomes and/or miRNA.

Abstract: The invention features methods and apparatus for producing lipid nanoparticles. Methods of the invention include injecting a lipid solution into an aqueous solution at an automated rate (e.g., a rate controlled by a servo pump). The invention provides methods and apparatus for making lipid nanoparticles possessing a wide range of lipid components and hydrophilic encapsulants, including nucleic acids (e.g., mRNA). Also provided are nanoparticles and compositions thereof made by methods and apparatus of the invention.

Abstract: The invention features methods and apparatus for concentrating a nucleic acid. Methods of the invention include providing an initial suspension of a nucleic acid and an initial liquid, contacting the initial suspension with a housing having a filter that does not pass the nucleic acid, pressurizing the housing to produce a filtrate and a nucleic acid retentate from the initial solution, and detecting the volume of the nucleic acid retentate. Apparatus of the invention include a chamber configured to hold a filter housing containing a nucleic acid suspension, a pressure source to filter the suspension, and a detector to depressurize the housing upon detecting the volume reaching a predetermined threshold. The methods and apparatus described herein are useful in filtering, concentrating, and reconstituting nucleic acids, such as mRNA, in processes such as complete buffer replacement.

Abstract: The invention features methods and apparatus for purifying a sample including an RNA (e.g., an RNA having a poly(A) sequence, such as an mRNA). Methods of the invention involve adding a liquid to a sample container including the sample and a solid affinity material (e.g., oligo(dT) or hydroxyapatite), agitating the contents of the sample, removing the liquid, and eluting the RNA of interest from the sample container. The apparatus of the invention include a sample container and one or more components such as a reservoir, collection container, pressure generating source, valve assembly, and agitation mechanism. Certain apparatus include a rotatable magnet or magnetizable component. The methods and apparatus described herein are useful in providing RNA (e.g., RNA having a poly(A) sequence, such as mRNA) in high yield and with high purity using less solvent than conventional purification techniques.

Abstract: Wall panels for a corrugated paper board container have bulge control lines formed therein to control the bending of the wall panels as the container is loaded with contents, such as liquid containing contents. The bulge control lines are configured to control the bending of the wall panels to mitigate the possibility of undesirable spouting that makes the containers more difficult to store.

Abstract: Methods are disclosed herein for treating glaucoma in a subject. In some embodiments, the methods increase retinal ganglion cell survival. The disclosed method use exosomes and/or miRNA.

Abstract: A method for determining a desired intake manifold pressure of an internal combustion engine by means of an iterative method, wherein a cylinder charge is determined for an intake manifold pressure iterated during the iterative method, and the desired intake manifold pressure is determined as a function of the cylinder air charge that has been determined. In addition, a control device for carrying out the method is provided.

Abstract: A method for processing a plurality of sheet material includes receiving a plurality of sheets of material, transporting, via a transport mechanism, the plurality of sheets, one sheet at a time, past a first image capture device, obtaining, via a first image capture device, a first image of a first set of one or more of the plurality of sheets while being transported past the first image capture device, and determining, via at least one processor, a first caliper measurement of the first set of one or more of the plurality of sheets based on the first image of each of the first set.

Abstract: A method for processing a plurality of sheet material includes receiving a plurality of sheets of material, transporting, via a transport mechanism, the plurality of sheets, one sheet at a time, past a first image capture device, obtaining, via a first image capture device, a first image of a first set of one or more of the plurality of sheets while being transported past the first image capture device, and determining, via at least one processor, a first caliper measurement of the first set of one or more of the plurality of sheets based on the first image of each of the first set.

Abstract: With the machine, workpieces are machined as they pass through the machine. At least one of the spindles of the machine is coupled with an adjusting unit with which the spindle, for producing a contour on the workpiece, can be adjusted transverse to the throughfeed direction of the workpiece during workpiece throughfeed. The adjustment of the adjusting unit is realized as a function of the advancing speed of the workpiece and/or the workpiece position upon throughfeed of the workpiece. The adjusting unit has such a stiffness and/or low clearance and/or positioning precision that the end products machined from the workpieces after exiting from the machine can be used without further post-machining. The end products have such a geometry precision and surface quality that further post-machining is no longer required.

Abstract: A method for processing a plurality of sheet material includes receiving a plurality of sheets of material, transporting, via a transport mechanism, the plurality of sheets, one sheet at a time, past a first image capture device, obtaining, via a first image capture device, a first image of a first set of one or more of the plurality of sheets while being transported past the first image capture device, and determining, via at least one processor, a first caliper measurement of the first set of one or more of the plurality of sheets based on the first image of each of the first set.

Abstract: Aircraft, vibration isolation assemblies, and methods of assembling vibration isolation assemblies are provided. An aircraft includes a vibration isolation assembly. The vibration isolation assembly includes a mounting track, a plate, a clamp block, a vibration isolator, and a support fitting. The mounting track defines a cavity and includes flanges that define an opening to the cavity. The plate is configured to be positioned on the flanges outside of the cavity. The clamp block is configured to be fastened to the plate under the flanges of the mounting track within the cavity. The vibration isolator is configured to be laterally constrained by the clamp block within the cavity. The support fitting is configured to be secured to the vibration isolator outside of the cavity.

Abstract: Wall panels for a corrugated paper board container have bulge control lines formed therein to control the bending of the wall panels as the container is loaded with contents, such as liquid containing contents. The bulge control lines are configured to control the bending of the wall panels to mitigate the possibility of undesirable spouting that makes the containers more difficult to store.

Abstract: Aircraft, vibration isolation assemblies, and methods of assembling vibration isolation assemblies are provided. An aircraft includes a fuselage, a mounted component disposed within the fuselage, and a vibration isolation assembly disposed within the fuselage and mounting the mounted component to the fuselage. The vibration isolation assembly includes a mounting track, an inner member, a support fitting, and an elastomer. The mounting track defines a cavity and an opening. The inner member has a post and a flange, where the flange is disposed in the cavity. The support fitting is secured to the post and the mounted component. The elastomer is disposed between the flange and the mounting track within the cavity.

Abstract: ‘BFC68’ is a distinctive variety of Thuja plicata×standishii which is characterized by the combination of an overall smaller plant size, dense foliage at the plant's apex where normally there are few branches or branchlets, a higher degree of branching among first, secondary and tertiary branchlets, a pendulous attitude of the terminal portion of lateral branches and all branchlets which creates a soft, feathered foliage texture, and stability and uniformity of traits through successive cycles of asexual propagation.