Abstract: The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated.

Abstract: The invention relates to optimised micro-organism strains for the biotransformation production of molecules having NADPH-consuming biosynthetic pathways. The inventive strains can be used in NADPH-consuming biotransformation methods. Said strains are characterized in that one or more NADPH-oxidizing activities are limited.

Abstract: The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.

Abstract: The invention concerns a method for analysing the intracellular chemical state of living cells by nuclear magnetic resonance (NMR). Said method comprises a step which consists in preparing the cell sample and a step which consists in an NMR analysis of the sample, both steps being carried out at very low temperature so as to freeze the biochemical state of the living cells to obtain specific and accurate reproducible measurements of the cellular functioning in given conditions. The invention also concerns the device coupled to the NMR spectrometer for implementing the method of analysis.

Abstract: The present invention is a waste-tire foundation bag. Stackable bags suitable for containing soil are filled with 50% waste tire portions and 50% soil. The bag openings are closed to prevent the contents from falling out. The bags are stacked in piles in a building-foundation excavation pit. Concrete is poured over and between the piles, whereby the solidified concrete foundation rests on the piles of waste-tire bags.

Abstract: The invention relates to optimised micro-organism strains for the biotransformation production of molecules having NADPH-consuming biosynthetic pathways. The inventive strains can be used in NADPH-consuming biotransformation methods. Said strains are characterised in that one or more NADPH-oxidising activities are limited.

Abstract: The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated.

Abstract: The invention relates to a method for the preparation of evolved microorganisms which permit a modification of metabolic pathways, comprising the following steps: a) production of a modified microorganism by genetic modification of initial microorganism cells such as to inhibit the production of or the consumption of a metabolite when the microorganism is cultivated in a defined medium which also affects the capacity of the microorganism for growth, b) culture of the modified microorganisms to induce evolution in said cells, c) selection of the cells of modified microorganisms which are capable of developing in the defined medium. The invention also relates to the strains of evolved microorganisms, the genes evolved which code for evolved proteins and the use of said evolved microorganisms, genes or proteins in a biotransformation method.

Abstract: The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.

Abstract: The invention concerns a method for analysing the intracellular chemical state of living cells by nuclear magnetic resonance (NMR). Said method comprises a step which consists in preparing the cell sample and a step which consists in an NMR analysis of the sample, both steps being carried out at very low temperature so as to freeze the biochemical state of the living cells to obtain specific and accurate reproducible measurements of the cellular functioning in given conditions. The invention also concerns the device coupled to the NMR spectrometer for implementing the method of analysis.