Abstract: A new device and methods that allow for improved sequestration and preservation of harvested analytes and biomolecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing.

Abstract: A device configured to capture analytes from a large volume of fluid (e.g. more than 2 mL of urine, broth, or water), concentrate those analytes, and elute them into a small volume (e.g. 100 uL) for subsequent analysis is described. Use of the apparatus for more than 100×-1000× concentration of analytes. Hydrogel particles are specially engineered and designed to capture and retain specific analytes from the fluid and then exclude other, unwanted analytes. The device has three pieces, including a fluid filtration cup, a waste collection chamber, and an elution collection chamber.

Abstract: A system and method configured to achieve hydrogel particle treatment for the reduction of metastatic burden in an immunocompetent syngeneic mouse model is described. The system seeks to achieve methodic optimization for the extraction and purification of extracellular vesicles (EVs) from cultured cells as well as from fresh breast cancer interstitium. Similarly, the system provides for lymph node remodeling by human breast cancer EVs in a humanized mouse model. Hydrogel particles are employed to convey cytokine releasing and EV-displaying treatment to afflicted bodies.

Abstract: A new device and methods that allow for improved sequestration and preservation of harvested analytes and biomolecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing.

Abstract: A new device and methods that allow for improved sequestration and preservation of harvested analytes and bio-molecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing.