Abstract: The subject matter disclosed herein is generally directed to modulation of genes and pathways that drive differentiation of LGR5+ stem cells. The methods and compositions can be used to treat diseases associated with aberrant epithelial barrier function. Using novel screening methods Applicants have identified compounds that increase Paneth cell differentiation. The compounds can be used to treat diseases associated with stem cell differentiation.

Abstract: Described in certain example embodiments herein are systems, methods, and uses thereof for high-throughput in vitro evaluating multiple test compounds in parallel for biological or pharmacological functions. In certain embodiments, the system allows the selection of a subset of test compounds from a group of test compounds to form an optimized pool, and methods are provided to use such optimized pool of test compounds to identify and validate therapeutic agents for treating diseases and driving guided differentiation of stem cells into desired types of cells. The systems described herein can provide, for example, a cost-effective and high-quality high-throughput approach for drug screening.

Abstract: The subject matter disclosed herein is generally directed to modulation of genes and pathways that drive differentiation of LGR5+ stem cells. The methods and compositions can be used to treat diseases associated with aberrant epithelial barrier function.

Abstract: Data elements are stored at a plurality of nodes. Each data element is a member data element of one of a plurality of layouts. Each layout indicates a unique subset of nodes. All member data elements of the layout are stored on each node in the unique subset of nodes. A stored dependency list includes every layout that has member data elements. The dependency list is used to determine availability of data elements based on ability to access data from nodes from the plurality of nodes.

Abstract: Data elements are stored at a plurality of nodes. Each data element is a member data element of one of a plurality of layouts. Each layout indicates a unique subset of nodes. All member data elements of the layout are stored on each node in the unique subset of nodes. A stored dependency list includes every layout that has member data elements. The dependency list is used to determine availability of data elements based on ability to access data from nodes from the plurality of nodes.