Abstract: Methods for incorporating and/or immobilizing security markers by exposure to an electromagnetic pulse, such as for instance a LASER pulse, to produce a marked object that can be authenticated only by using proprietary biological, chemical or physical analysis, the method includes: exposing a surface of the object to be marked to an electromagnetic pulse to activate the surface or a coating on the surface, and exposing the surface to a detectable marker molecule and thereby immobilizing the detectable marker molecule, such as a biological marker molecule, e.g. DNA on the surface of the object. The method of marking the object may include exposing a photo-polymerizable monomer on at least a portion of the surface of an object to be marked to a LASER pulse to initiate a reaction to cross link the photo-polymerizable monomer binding, trapping or encapsulating a detectable marker on the surface of the object.

Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.