Abstract: A composition for tendon and ligament regeneration includes a biologically compatible hydrogel suspension having spherical droplets. The spherical droplets have a diameter of about 100 ?m to about 300 ?m and a plurality of cells. The cells include marrow-derived mesenchymal stem cells (MSCs) with an average viability of about 90% or greater and cells that retain their stemness. A method for tendon and ligament regeneration includes forming the composition, purifying the composition, retrieving the composition, and administering the composition via injection into an injury site of injured tissue.

Abstract: A hydrogel particle encapsulation and suspension media removal method includes combining hydrogel materials, which include a hydrogel polymer, a linker, a cargo suspension, and a suspension media. The hydrogel materials are mixed to form a hydrogel-suspension media mixture. The mixed hydrogel materials are polymerized to form hydrogel particles having encapsulated cargo. The suspension media is substantially removed from the hydrogel-suspension media mixture by: adding a density adjusting material, forming a three-phase solution that includes the density adjusting material settled between the hydrogel particles and the suspension media, centrifuging the three-phase solution, agitating the three-phase solution, centrifuging the three-phase solution, and retrieving the hydrogel particles lacking the suspension media.

Abstract: A composition for tendon and ligament regeneration includes a biologically compatible hydrogel suspension having spherical droplets. The spherical droplets have a diameter of about 100 ?m to about 300 ?m and a plurality of cells. The cells include marrow-derived mesenchymal stem cells (MSCs) with an average viability of about 90% or greater and cells that retain their stemness. A method for tendon and ligament regeneration includes forming the composition, purifying the composition, retrieving the composition, and administering the composition via injection into an injury site of injured tissue.

Abstract: Embodiments of the present disclosure generally relate to methods and compositions for controlling cellular expression. More specifically, embodiments described herein relate to hydrogel-encapsulated/dispersed cells, methods of forming hydrogel-encapsulated/dispersed cells, and methods of using hydrogel-encapsulated/dispersed cells for controlling production of, for example, secretomes. In an embodiment, a composition for controlling production of secretomes is provided. The composition includes, a hydrogel comprising, in polymerized form, one or more photoreactive monomers and a thiol linker, wherein at least one of the one or more photoreactive monomers comprises a methylene functional group; and one or more cells dispersed or encapsulated within the hydrogel.

Abstract: Embodiments of the present disclosure generally relate to apparatus for isolating biological material, processes for fabricating such apparatus, and processes for using such apparatus. In an embodiment, an apparatus for isolating a biological material is provided. The apparatus includes a fluidic channel disposed over a portion of a substrate. The apparatus further includes a hydrogel structure disposed in the fluidic channel, the hydrogel structure comprising a plurality of wells, wherein each well of the plurality of wells has a diameter from about 1 ?m to about 500 ?m, the hydrogel structure comprising, in polymerized form, one or more photoreactive monomers.

Abstract: Embodiments of the present disclosure generally relate to compositions comprising hydrogel-encapsulated/dispersed beta cells, compositions comprising hydrogel-encapsulated/dispersed beta-cell spheroids, processes for forming such compositions, and uses of the compositions. In an embodiment, a composition is provided that includes a first component comprising a hydrogel, the hydrogel comprising, in polymerized form, one or more photoreactive monomers and a thiol linker. The composition further comprises a second component comprising a plurality of beta cells dispersed or encapsulated within the hydrogel.

Abstract: Embodiments of the present disclosure generally relate to compositions that include hydrogel-encapsulated/dispersed cells, compositions including hydrogel-encapsulated/dispersed cells, and to processes for forming such hydrogel-encapsulated/dispersed cells and compositions thereof. The compositions can be used for, e.g., therapeutic applications. In some examples, the hydrogel-encapsulated/dispersed cells are formed using photoreactive groups chemically attached to polyethylene glycol to form a material which, upon exposure to a desired wavelength or wavelength range of light, reacts to form a cross-linked hydrogel network.