Abstract: A recombinant spider silk protein comprises an NT domain, a REP domain and a CT domain. The REP domain comprises a set of domains according to the formula pA1-pG-pA2. pG represents a glycine-rich domain and pA1 and pA2 represent alanine-rich domains. One of pA1 and pA2 is a poly-alanine domain and the other of pA1 and pA2 is a poly-alanine domain having every third or fourth alanine residue replaced by an isoleucine residue or a valine residue. Spider silk spun from the recombinant spider silk proteins of the invention have improved mechanical properties.

Abstract: The present embodiments relate to antibody binding nanofibrils obtainable by co-fibrillation of carrier proteins and carrier-Z fusion proteins at a molar ratio selected within an interval of from 1:0.20 to 1:0.90. The antibody binding nanofibrils have extremely high antibody binding capacity and can thereby be used in various applications, such as antibody purification, and detection of biomarkers in point of care or laboratory diagnosis applications. (FIG. 6).

Abstract: The present embodiments relate to antibody binding nanofibrils obtainable by co-fibrillation of carrier proteins and carrier-Z fusion proteins at a molar ratio selected within an interval of from 1:0.20 to :0.90. The antibody binding nanofibrils have extremely high antibody binding capacity and can thereby be used in various applications, such as antibody purification, and detection of biomarkers in point of care or laboratory diagnosis applications.

Abstract: A method of allocating hydraulic fluid between actuators in a machine accepts a first command to provide a first requested fluid flow to a first actuator, wherein the first actuator is a bounded actuator such as a steering actuator, and a second command to provide a second requested fluid flow to a second actuator. The system adjusts the first and second commands to produce adjusted first and second commands corresponding to adjusted first and second fluid flows, such that the sum of the adjusted first and second fluid flows is less than or equal to a maximum available flow and the adjusted first fluid flow meets or exceeds the lesser of the first requested fluid flow and a threshold curve that is a function of engine speed or other variable.

Abstract: A method of allocating hydraulic fluid between actuators in a machine accepts a first command to provide a first requested fluid flow to a first actuator, wherein the first actuator is a bounded actuator such as a steering actuator, and a second command to provide a second requested fluid flow to a second actuator. The system adjusts the first and second commands to produce adjusted first and second commands corresponding to adjusted first and second fluid flows, such that the sum of the adjusted first and second fluid flows is less than or equal to a maximum available flow and the adjusted first fluid flow meets or exceeds the lesser of the first requested fluid flow and a threshold curve that is a function of engine speed or other variable.