Abstract: The present invention provides for improved antimicrobial susceptibility testing and more specifically for improved rapid antimicrobial susceptibility testing of clinical samples for efficient and versatile analysis and reliable results.

Abstract: Systems and methods for antimicrobial susceptibility testing (AST) are provided in which variances in anionic charge of microbes are taken into account. Cationic surfactants may be used to sensitize otherwise resistant microorganisms to polycationic antibiotics, such as polymyxins. Since microorganisms gain polycationic antibiotic resistance through mutations that decrease surface anionic charge, the susceptibility of a microorganism to a polycationic antibiotic may be indicative of its surface charge. In order to enable electrostatic interactions with the microorganism surface, a cationic surfactant may be applied to increase the anionic charge of the microorganism.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: This disclosure relates to antimicrobial susceptibility testing. More specifically, this disclosure relates to improved rapid antimicrobial susceptibility testing of clinical samples for efficient and versatile analysis and reliable results.

Abstract: The present invention provides for improved antimicrobial susceptibility testing and more specifically for improved rapid antimicrobial susceptibility testing of clinical samples for efficient and versatile analysis and reliable results.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: Systems and methods for antimicrobial susceptibility testing (AST) are provided in which variances in anionic charge of microbes are taken into account. Cationic surfactants may be used to sensitize otherwise resistant microorganisms to polycationic antibiotics, such as polymyxins. Since microorganisms gain polycationic antibiotic resistance through mutations that decrease surface anionic charge, the susceptibility of a microorganism to a polycationic antibiotic may be indicative of its surface charge. In order to enable electrostatic interactions with the microorganism surface, a cationic surfactant may be applied to increase the anionic charge of the microorganism.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: The use of metabolic probes is well-established for determining cell viability and assessing drug cytotoxicity. Resazurin-based formulations, in particular, have found utility for determining susceptibilities of microorganisms to antimicrobials, specifically through their use in antibiotic susceptibility testing (AST). There is a strong need currently to shorten AST durations, thus resazurin formulations that produce signals earlier are advantageous. This need results from the slow state of clinical microbiology testing, which may leave patients exposed to unnecessary or ineffective broad-spectrum agents for prolonged periods of time. Another slow microbiology test is Gram staining, still most often performed manually in sequential steps. Speeding Gram typing, preferably with an automated platform, would also speed time-to-results and decrease manual workloads on medical technologists in clinical microbiology laboratories.

Abstract: The present disclosure provides novel methods for microbiological sample preparation from patients that yield high quality input material for antimicrobial susceptibility testing (AST) without the need for plate culture. Certain methods of this disclosure comprise multiple separation steps to purify microbial samples for use in ASTs.

Abstract: Nanoparticles for use in assay methods for detecting analytes in samples, which comprise a signal inducing agent, e.g. a transition-metal catalyst or a chemiluminophore, a chemiluminophore precursor, a soluble absorber, or a soluble absorber precursor. After binding to an analyte, the nanoparticle is dissociated by a chemical or physical trigger, e.g. an organic solvent or ultrasound, to release the signal inducing agent, which releases a detectable signal via a physical or chemical reaction. The nanoparticles comprising a chemiluminophore, a chemiluminophore precursor, a soluble absorber, or a soluble absorber precursor can also effect chemical reactions that serve as signal amplifiers.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.