Abstract: Control Devices are disclosed including RNA destabilizing elements (RDE), RNA control devices, and destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) or other transgenes in eukaryotic cells. Multicistronic vectors are also disclosed for use in engineering host eukaryotic cells with the CARs and transgenes under the control of the control devices. These control devices can be used to optimize expression of CARs in the eukaryotic cells so that, for example, effector function is optimized. CARs and transgene payloads can also be engineered into eukaryotic cells so that the transgene payload is expressed and delivered after stimulation of the CAR on the eukaryotic cell.

Abstract: The present invention relates generally to the field of RNA Control Devices and/or destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) in eukaryotic cells. The present invention also relates to split CARs (Side-CARs) in eukaryotic cells. More specifically, the present invention relates to DEs, RNA Control Devices, and/or side-CARs combined with Chimeric Antigen Receptors to make small molecule actuatable CAR polypeptides. The present invention also relates to DE-CARs, Smart CARs (Smart=small molecule actuatable RNA trigger), Smart-DE-CARs, and/or Side-CARs for use in the treatment of disease.

Abstract: In an aspect, the present invention relates generally to RNA control devices, destabilizing elements (“DE”), Solute Carrier Transporters (“SLC”), and/or control regions where some or all of these control elements are ligand matched. In another aspect, the present invention relates to ligand matched control regions, SLCs and/or control devices that produce control systems with desired properties. In a further aspect, the present invention relates to use of these control systems to control expression of transgenes in eukaryotic cells including, for example, stem cells, hematopoietic cells, and host cells for gene therapy.

Abstract: Control Devices are disclosed including RNA destabilizing elements (RDE), RNA control devices, and destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) or other transgenes in eukaryotic cells. Multicistronic vectors are also disclosed for use in engineering host eukaryotic cells with the CARs and transgenes under the control of the control devices. These control devices can be used to optimize expression of CARs in the eukaryotic cells so that, for example, effector function is optimized. CARs and transgene payloads can also be engineered into eukaryotic cells so that the transgene payload is expressed and delivered after stimulation of the CAR on the eukaryotic cell.

Abstract: The present invention relates generally to the field of RNA Control Devices and/or destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) in eukaryotic cells. The present invention also relates to split CARs (Side-CARs) in eukaryotic cells. More specifically, the present invention relates to DEs, RNA Control Devices, and/or side-CARs combined with Chimeric Antigen Receptors to make small molecule actuatable CAR polypeptides. The present invention also relates to DE-CARs, Smart CARs (Smart=small molecule actuatable RNA trigger), Smart-DE-CARs, and/or Side-CARs for use in the treatment of disease.

Abstract: The present invention relates generally to the field of making novel antigen binding domains against infectious diseases. The present invention also relates to novel CARs that utilize the novel antigen binding domains as an extracellular element. The present invention also relates to use of the novel antigen binding domains as therapeutic agents.

Abstract: The present invention relates generally to the field of RNA Control Devices and/or destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) in eukaryotic cells. The present invention also relates to split CARs (Side-CARs) in eukaryotic cells. More specifically, the present invention relates to DEs, RNA Control Devices, and/or side-CARs combined with Chimeric Antigen Receptors to make small molecule actuatable CAR polypeptides. The present invention also relates to DE-CARs, Smart CARs (Smart=small molecule actuatable RNA trigger), Smart-DE-CARs, and/or Side-CARs for use in the treatment of disease.

Abstract: In an aspect, the present invention relates generally to the field of treating disease with CAR devices, Smart CAR devices, DE CAR devices, and/or Smart-DE CAR devices. The present invention also relates generally to the genetic modification of cytotoxic T-lymphocytes to reduce target cell killing by apoptosis and/or increase production of lytic proteins at desired times. In an aspect, the invention relates to the use of these genetically modified T-lymphocytes and/or natural killer cells with CAR devices, Smart CAR devices, DE CAR devices, and/or Smart-DE CAR devices to enhance the immune response against a disease.

Abstract: Systems, devices, and methods to treat a urinary bladder are disclosed. An expandable member is introduced and expanded in the urinary bladder to appose one or more elongate conductors on the outer surface of the expandable member against the inner wall of the urinary bladder. The one or more elongate conductors are used to create a predetermined pattern of electrically isolated tissue regions having reduced electrical propagation such that electrical propagation through the urinary bladder as a whole is reduced. A mucus layer may be removed from the inner bladder wall prior to the ablation. Ablation may be regulated by impedance measurement with the one or more elongate conductors. The urinary bladder may be filled with a fluid to facilitate the impedance measurement.

Abstract: The present invention relates generally to the field of RNA Control Devices and/or destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) in eukaryotic cells. The present invention also relates to split CARs (Side-CARs) in eukaryotic cells. More specifically, the present invention relates to DEs, RNA Control Devices, and/or side-CARs combined with Chimeric Antigen Receptors to make small molecule actuatable CAR polypeptides. The present invention also relates to DE-CARs, Smart CARs (Smart=small molecule actuatable RNA trigger), Smart-DE-CARs, and/or Side-CARs for use in the treatment of disease.

Abstract: The invention provides materials and methods for forming coatings on substrates. The coatings are durable and resistant to damage from environmental, chemical, thermal, and/or radiative sources. In some embodiments, the coatings comprise bilayers of electrostatically charged materials. The bilayers are created by alternately applying solutions comprising water-soluble, electrostatically charged materials. Durability is imparted to the coatings by the formation of crosslinks that are formed within and between layers after deposition of the coatings.

Abstract: Traditional solar control applications rely on thin metal films to reflect EM radiation with wavelengths longer than that of the visible spectrum. Unfortunately such films also block radiation in cellular, GPS, and radio frequency bands. In one aspect, the disclosure provides a selectively-blocking filter that uses one or more optical filters tuned to a specific range(s) of wavelengths (e.g. for blocking IR radiation), while readily transmitting other wavelengths (e.g. both visible light and cellular/GPS signals). The filters can be manufactured on both flexible and rigid substrates.

Abstract: The disclosure provides materials, apparatuses, and methods for making multilayer coatings with a high degree of efficiency and control. In some aspects, for example, coatings are described having multiple layers of nanoparticles and a polyelectrolyte, wherein the nanoparticles form tightly packed monolayers. The interface between monolayers may include polyelectrolyte material. One or more aspects of such monolayers and interfaces are controllable.

Abstract: The invention provides materials and methods for forming coatings on substrates. The coatings are durable and resistant to damage from environmental, chemical, thermal, and/or radiative sources. In some embodiments, the coatings comprise bilayers of electrostatically charged materials. The bilayers are created by alternately applying solutions comprising water-soluble, electrostatically charged materials. Durability is imparted to the coatings by the formation of crosslinks that are formed within and between layers after deposition of the coatings.

Abstract: The disclosure provides methods and materials for preparing bridging films. In one aspect, the bridging films are non-porous and are suitable for protecting adjacent porous films. For example, the bridging films contact a porous film and protect the porous film from transfer of gases and/or liquids into the pores of the porous film. In another example, bridging films protect the porous film from abrasion.

Abstract: Traditional solar control applications rely on thin metal films to reflect EM radiation with wavelengths longer than that of the visible spectrum. Unfortunately such films also block radiation in cellular, GPS, and radio frequency bands. In one aspect, the disclosure provides a selectively-blocking filter that uses one or more optical filters tuned to a specific range(s) of wavelengths (e.g. for blocking IR radiation), while readily transmitting other wavelengths (e.g. both visible light and cellular/GPS signals). The filters can be manufactured on both flexible and rigid substrates.

Abstract: The disclosure provides materials, apparatuses, and methods for making multilayer coatings with a high degree of efficiency and control. In some aspects, for example, coatings are described having multiple layers of nanoparticles and a polyelectrolyte, wherein the nanoparticles form tightly packed monolayers. The interface between monolayers may include polyelectrolyte material. One or more aspects of such monolayers and interfaces are controllable.

Abstract: Durable coatings and methods for producing the same are provided, where the coatings may include porous coatings encapsulated with a hardening solution that permeates into the porous structure of the film prior to curing. Curing of the hardening solution within the film provides for a durable coating having sufficient durability for use in many different applications, such as optical applications. Any convenient porous coatings may be used in the subject methods. Also provided are methods for forming a coating formulation, where the formulation includes porous coating particles dispersed in a carrier and the porous coating particles may be optionally encapsulated with a hardening solution prior to dispersion.

Abstract: The invention provides optical elements having a substrate with a textured surface, and a coating disposed on the textured surface. The coating is a multi-layer optical coating that provides desirable optical properties for the optical element. The coating is conformally disposed on the textured surface of the substrate. The invention also provides methods for making and methods for using such optical elements.

Abstract: The invention provides methods for modifying one or more properties of porous thin films. In such methods, a formulation comprising a reactive species is applied to the porous thin film and allowed to crosslink. In some embodiments, the crosslinked network thus formed imparts increased mechanical strength and wear resistance to the porous thin films.