Abstract: Provided herein are gene repressor systems comprising fusion proteins, such as fusion proteins comprising a DNA binding domain such as a TALE, zinc finger or catalytically-dead CRISPR protein and guide nucleic acid (gRNA), which are useful in the repression of a proprotein convertase subtilisin kexin Type 9 (PCSK9) gene. Also provided are methods of using such systems to repress transcription of PCSK9.

Abstract: Provided herein are gene repressor systems comprising fusion proteins, such as fusion proteins comprising a DNA binding domain such as a TALE, zinc finger or catalytically-dead CRISPR protein and guide nucleic acid (gRNA), which are useful in the repression of a proprotein convertase subtilisin/kexin Type 9 (PCSK9) gene. Also provided are methods of using such systems to repress transcription of PCSK9.

Abstract: Tungsten precursors with high purity and methods for purifying tungsten precursors are provided. The method for purifying a precursor may comprise at least one of the following steps: obtaining a source vessel containing WCl4, WOCl4, and one of WCl5 or WCl6; separating the WCl5 or the WCl6 from at least a first portion of the WOCl4; separating the WCl5 or the WCl6 from at least a second portion of the WOCl4; recovering a precursor in a collection vessel; or any combination thereof.

Abstract: Nuclear fuel rods are disclosed. The nuclear fuel rods include a substrate and a chromium alloy coating layer applied to the substrate. The chromium alloy coating layer comprises chromium (Cr); a element or compound selected from the group consisting of yttrium (Y), lanthanum (La), thorium (Th), zirconium (Zr), titanium (Ti), hafnium (I-lf), molybdenum (Mo), tungsten (W), vanadium (V), rhenium (Re), ruthenium (Ru), cobalt (Co), aluminum (Al), carbides, borides, intermetallics, and combinations thereof; and interstitial elements up to 1500 ppm, wherein carbon (C), oxygen (O), and nitrogen (N) are each 500 ppm or less.

Abstract: The present disclosure describes compositions for reducing hypergranulation tissue and methods of their use. The compositions comprise bacitracin, polymyxin b, and triamcinolone.

Abstract: A method of forming a micro-lensed fiber optic plate. The method includes obtaining a fiber optic plate that has a plurality of fibers arranged in parallel. A first end of a fiber of the plurality of fibers can have a first numerical aperture. The fiber of the plurality of fibers includes a core material running along a length of the fiber that is circumscribed by a layer of cladding material. The method also includes creating a lensed surface at a second end of the fiber of the plurality of fibers. The second end is opposite the first end. The lensed surface of the fiber of the plurality of fibers can have a second numerical aperture that is different from the first numerical aperture.

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract: The present disclosure relates to medical delivery devices that include a barrel having an inner surface that is hydrophobic. The medical delivery device includes a barrel with a hydrophobic inner surface and a stopper that can provide air and liquid impermeability while also possessing on or more of: a low break loose force, a low average glide force, and a low glide force variation. The contact angle of the inner surface of the barrel is greater than 90°.

Abstract: Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes.

Abstract: Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes.

Abstract: Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes.

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract: A voice record integrator receives information to be acknowledged by a user such as a statement of facts, an electronic document, or other record. The system creates a document identifier that may be used to identify the information being acknowledged and uses the document identifier to present a voice script for the user to read. The system records the user reading the voice script and creates a voice record. The voice record may be stored and later used to verify the user's acknowledgement of the statement of facts, electronic document, or other record.

Abstract: A method of reducing heat produced by an internal combustion engine in a motorcycle. The method includes supplying fuel pulses to a combustion chamber at least once per engine cycle (consecutive engine cycles defining a series of consecutive fuel pulses), operating the motorcycle at a low speed condition, and withholding at least a portion of at least one fuel pulse from at least one subsequent engine cycle to the combustion chamber when operating the motorcycle at the low speed condition.

Abstract: A voice signature system includes a data store, an interface code segment operable to receive identification of information to be signed and a phone number, and a signature process code segment operable to place an outbound telephone call to the phone number and capture a voice recording. The voice signature system is operable to create a voice signature using the voice recording and the identification of information to be signed. A method for creating voice signatures includes receiving an identification of information to be signed, receiving a phone number, placing an outbound call to the phone number, creating a voice recording of at least a portion of the outbound call, and creating a voice signature using the identification of information to be signed and the voice recording.

Abstract: A method of reducing heat produced by an internal combustion engine in a motorcycle. The method includes supplying fuel pulses to a combustion chamber at least once per engine cycle (consecutive engine cycles defining a series of consecutive fuel pulses), operating the motorcycle at a low speed condition, and withholding at least a portion of at least one fuel pulse from at least one subsequent engine cycle to the combustion chamber when operating the motorcycle at the low speed condition.

Abstract: One aspect of the invention relates to a technique for effecting payment, and involves: presenting a portable device; and causing the portable device to output a pseudo payment code.

Abstract: A method is provided for separating people from direct access to personally identifiable information. The method involves use of a rules-based section which selectively blocks access to personally identifiable information where the access fails to comply with specified rules, and which selectively permits access to personally identifiable information where the access abides with the specified rules.

Abstract: A motorcycle comprising a frame, a front wheel coupled to the frame, and a rear wheel coupled to the frame. An engine is supported by the frame and is adapted to propel the motorcycle. An oil tank is supported by the frame and includes an inlet defining an inlet aperture having an inlet axis. An oil tank cap is rotatable about the inlet axis between an inserted position and a sealed position. The oil tank cap has a movable portion that is movable substantially along the inlet axis between a retracted position and an extended position.

Abstract: The invention presents a method and system for recording a detailed record or "transcript" of the acts, events and forensic circumstances related to a party's affirmation of an electronic document, transaction or event. The transcript is recorded in a data object made secure through the use of encryption and a checksum. The system directs a ceremony whereby the party affirming the document, transaction or event is required to undertake a series of steps in order to successfully complete the affirmation and have the affirmation recorded; thus participation in the ceremony must take place before an affirmation will be accepted. The steps of the controlled procedure serve to gather evidence to confirm specifics such as that the affirming party: i) is in fact the identified party; ii) understands that by entering affirming data, e.g.