Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides. The yeast cells include one or more heterologous nucleic acids that encode enzymes of a human milk oligosaccharide biosynthetic pathway. The yeast cells do not include a heterologous nucleic acid encoding a fucokinase. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering human milk oligosaccharides generated by the yeast cells.

Abstract: The present invention relates generally to targeted molecular agents (TMAs) directed to a particular organism or group of organisms and uses thereof. More particularly, the present invention provides TMAs having a targeting moiety which comprises a natural or induced auxotrophic requirement of the particular organism as a vehicle for directing an agent linked to the moiety to be delivered to the target organism. The TMAs of the present invention are useful for targeting molecules such as antimicrobial agents and diagnostic agents to selected organisms.

Abstract: A method an apparatus for simultaneously dispensing a plurality of viscous materials, such as dentifrice pastes, produces a striped outer layer of a first material surrounding a core of a second viscous material. The apparatus includes a collapsible tube or pump-type dispenser containing a first viscous material and a second viscous material of a contrasting color in contact with the first material so that both materials are dispensed simultaneously. The tube or dispenser includes an outlet nozzle having a shaped outlet capable of scraping and removing a portion of the outer layer of viscous material to dispense the core material having stripes of the outer material. The nozzle is capable of forming a plurality of fine pin stripes on the core material.