Abstract: Card guide misalignment prevention apparatuses and methods are disclosed for preventing misalignment of cards into a chassis. According an embodiment of one apparatus, a card guide system for facilitating insertion of printed circuit boards (PCBs) into a telecommunications equipment chassis is provided. The card guide system includes a support member for supporting a plurality of pairs of parallel rails extending outward from the support member and across portions of a surface of the support member. Each pair of parallel rails forms a card guide for guiding a PCB across the surface to an electrical connector. The pairs of parallel rails are spaced from each other on the surface. The card guide system also include a plurality of misalignment prevention members extending outward from the surface of the support member between adjacent card guides for preventing PCB misalignment. The support member, rails, and misalignment prevention members are of unitary construction.