Abstract: The invention relates to a method for determining calving characteristics in bovine subjects, wherein calving characteristics comprise stillbirth, calving difficulty and calf size at birth, which are all economically important factors. In particular, the method of the invention involves identification of genetic markers and/or Quantitative Trait Locus (QTL) for the determination of calving characteristics in a bovine subject. The determination of calving characteristics involves resolution of the specific microsatellite status. Furthermore, the invention relates to a diagnostic kit for detection of genetic marker(s) associated with calving characteristics. The method and kit of the present invention can be applied for selection of bovine subjects for breeding purposes. Thus, the invention provides a method of genetically selecting bovine subjects with calving characteristics that will yield cows less prone to stillbirth, calving difficulties and undesired calf size at birth.