Abstract: An exemplary system, apparatus, method and computer-accessible medium for determining information regarding a sample(s), can be provided, which can include, for example, a source arrangements(s) which can provide a first radiation(s), whose intensity can vary over time, to the sample(s), a detector arrangement(s) which can be configured to receive a second radiation(s) from the sample(s) based on the first radiation(s) and a computer arrangement(s) which can be configured to simultaneously determine the information regarding the sample(s) at a plurality of frequencies of the second radiation(s).

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.