Patents by Inventor Bernard M. Churchill
Bernard M. Churchill has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240068008Abstract: The present invention relates generally to materials and methods for detection of bacteria, and for testing and determination of antibiotic susceptibility of bacteria in specimens of bodily fluid and other samples. The invention also relates to materials and methods for monitoring the physiological response of bacteria to antimicrobial agents, and for reducing background and increasing sensitivity of assays that involve the detection and/or measurement of RNA, such as rRNA. The invention provides kits comprising an RNase packaged for use in the methods described herein.Type: ApplicationFiled: August 21, 2023Publication date: February 29, 2024Inventors: David A. Haake, Daniel Gussin, Gabriel K. Monti, Bernard M. Churchill, Colin W. Halford
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Publication number: 20190376116Abstract: The present invention relates generally to materials and methods for detection of bacteria, and for testing and determination of antibiotic susceptibility of bacteria in specimens of bodily fluid and other samples. The invention also relates to materials and methods for monitoring the physiological response of bacteria to antimicrobial agents, and for reducing background and increasing sensitivity of assays that involve the detection and/or measurement of RNA, such as rRNA. The invention provides kits comprising an RNase packaged for use in the methods described herein.Type: ApplicationFiled: December 5, 2017Publication date: December 12, 2019Inventors: David A. Haake, Daniel Gussin, Gabriel K. Monti, Bernard M. Churchill, Colin W. Halford
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Patent number: 10370728Abstract: Described are probes and methods for detecting antibiotic susceptibility of a specimen. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.Type: GrantFiled: May 3, 2013Date of Patent: August 6, 2019Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE UNITED STATES OF AMERICA REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRSInventors: David A. Haake, Colin Halford, Jane T. Babbitt, Bernard M. Churchill
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Publication number: 20160160268Abstract: Described are methods for detecting susceptibility of a specimen to antibiotics, and particularly for enhancing such susceptibility testing for beta lactam antibiotics and antibiotics that bind to penicillin-binding proteins. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is mature ribosomal RNA or at the splice site between a pre-ribosomal RNA tail and mature ribosomal RNA. Performing the method in the presence and absence of an antibiotic permits determination of antibiotic susceptibility. Rapid susceptibility testing is enabled by the addition of the PBP2-specific antibiotic, amdinocillin.Type: ApplicationFiled: July 22, 2014Publication date: June 9, 2016Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNITED STATES GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRSInventors: David A. HAAKE, Bernard M. CHURCHILL, Colin HALFORD
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Publication number: 20150104789Abstract: Described are probes and methods for detecting antibiotic susceptibility of a specimen. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.Type: ApplicationFiled: May 3, 2013Publication date: April 16, 2015Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE UNITED STATES GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERAN AFFAIRSInventors: David A. Haake, Colin Halford, Jane T. Babbitt, Bernard M. Churchill
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Publication number: 20110027782Abstract: Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.Type: ApplicationFiled: May 26, 2010Publication date: February 3, 2011Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, U.S. GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRSInventors: DAVID A. HAAKE, BERNARD M. CHURCHILL, JOSEPH C. LIAO, MARC A. SUCHARD, YANG LI, MITRA MASTALI
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Patent number: 7763426Abstract: Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.Type: GrantFiled: May 1, 2007Date of Patent: July 27, 2010Assignees: The Regents of the University of California, The United States of America as represented by the Department of Veterans AffairsInventors: David A. Haake, Bernard M. Churchill, Joseph C. Liao, Marc A. Suchard, Yang Li, Mitra Mastali
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Publication number: 20080199863Abstract: Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.Type: ApplicationFiled: May 1, 2007Publication date: August 21, 2008Applicants: The Regents of the University of California, U.S. Government represented by the Dept. of Veterans AffairsInventors: David A. Haake, Bernard M. Churchill, Joseph C. Liao, Marc A. Suchard, Yang Li, Mitra Mastali
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Patent number: 6001060Abstract: A system for patient diagnostic testing includes the physiological data output is in a two dimensional graphical format. Optionally, the report includes imaging data. Color is a dimension provided to the graphical presentation of normal patient data in horizontal and vertical dimensions. Detrusor pressures are obtained. One detrusor pressure is the difference between average data points of the bladder and rectal pressures. The other is obtained by subtracting curve fitted rectal and bladder pressures. The displayed data is a presentation including bladder capacity information, pressure characteristics, and data as a function of volume such that bladder volume is indicated.Type: GrantFiled: May 30, 1997Date of Patent: December 14, 1999Assignee: Regents of the University of CaliforniaInventors: Bernard M. Churchill, Edward F. Wahl