Abstract: The invention relates to an electropolymerizable monomer, intended to be polymerized in aqueous solution, comprising a single electropolymerizable unit and an electron-donating group, characterized in that it also comprises at least one arm ionizable in aqueous solution. The invention also relates to the polymerization process, to the electroactive probe thus obtained and to the method for the detection of a target ligand in a biological sample.

Abstract: The invention relates to fluorescent polymers soluble in an aqueous solution carrying at least 5 fluorophores which are distributed on the polymer and which exhibit the following properties: the fluorophores are water-soluble, the fluorophores are not self-associated in water at a concentration of less than or equal to 10?4 mol/l, preferably at a concentration of less than or equal to 10?3 mol/l, the fluorophores which are free in the aqueous solution have a molar extinction coefficient of greater than 1000 M?1·cm?1, preferably greater than 5000 M?1·cm?1, the fluorophores which are free in the aqueous solution have a quantum yield of greater than 0.3, preferably greater than 0.6, said polymers having a fluorescence amplification factor of greater than or equal to 0.35 per kg/mol of polymer, preferably greater than 0.45 per kg/mol of polymer.

Abstract: The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sample. (I) wherein R1 is one type of oligonucleotide, Y is S or O, X is a spacer arm selected from —(CH2)—O—, —(CH2)<SB>P</SB>O—[(CH2)2—O]q—, —(CH2)r; —CO—NR?—(CH2)r—O—, —CH2r—NCH3—(CH2)r—O—, —CH2)rCO—NR?—[CH2)2—O]s—, —(CH2)rNCH3[(CH2)2—O]<SB>S</SB>—, R? is H or CH3, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r? is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r? and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

Abstract: The present invention relates to a method for simultaneously detecting hybridization reactions and immunoreactions in a sample which may contain target analytes consisting of at least one nucleic acid and of at least one other ligand that is different in nature, characterized in that it comprises the steps consisting in: (i) depositing a known amount of volume of the sample diluted in a reaction buffer, onto a capture surface pre-coated with the partners for capturing said target analytes, said capture partners consisting of at least one nucleic acid probe and at least one antiligand, (ii) reacting at a temperature of between 15° C. and 60° C. and (iii) visualizing the hybridization reactions and immunoreactions thus obtained, and also to the use of this method for detecting diseases of infectious or metabolic origin or viral origin, for the industrial diagnosis of the presence of bacteria, and for identifying and/or quantifying biological molecules.

Abstract: The invention relates to bi-functionalised metallocenes of general formula (I) where Me=a transition metal, preferably chosen from Fe, Ru and Os, Y and Z, when identical are selected from —(CH2)n—O—, (CH2)—O—[(CH2)2—O]P— and —(CH2)q—CONH—(CH2)r—O—, or Y=—(CH2)S—NH— and Z=—(CH2)t—COO—, n=a whole number from 3 to 6 inclusive, p=a whole number from 1 to 4 inclusive, q=a whole number from 0 to 2 inclusive, r=a whole number from 0 to 2 inclusive, s=a whole number from 2 to 5 inclusive, t=a whole number from 3 to 6 inclusive, R and R?=H atoms or are protective groups used in oligonucleotide and peptide synthesis, where at least one of R or R? is protective group used in oligonucleotide and peptide synthesis and R and R? are as defined below: (i) when Z and Y are selected from (CH2)n—O—, —(CH2)—O—[(CH2)2—O]p— and —(CH2)q—CONH—(CH2)r—O—, then R and R? are protective groups used in oligonucleotide synthesis and R is a group which can leave a free OH group after deprotection, preferably a photolabile group such as mono

Abstract: Viral material, in the isolated or purified state, in which the genome comprises a nucleotide sequence chosen from the group including sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, their complementary sequences and their equivalent sequences, in particular nucleotide sequences displaying, for any succession of 100 contiguous monomers, at least 50% and preferably at least 70% homology with the said sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, respectively, and their complementary sequences.

Abstract: The invention relates to composite particles comprising a hydrophobic polymer core and inorganic nanoparticles. Said hydrophobic polymer forms a polymer matrix inside which the inorganic nanoparticles are stabilized and distributed in a relatively homogenous manner. Said particles are at least partially surrounded by an amphophilic copolymer comprising a hydrophobic part and a hydrophilic part, said hydrophobic part being at least partially immobilized in the polymer matrix. The inventor also relates to the preparation method thereof, the conjugates comprising such particles and a ligand and the use of same in reactants and therapeutic compositions.

Abstract: The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.

Abstract: The invention provides viral material and nucleotide fragments associated with multiple sclerosis and/or rheumatoid arthritis for use in method of diagnosis, prophylaxis, and therapy.

Abstract: The present invention relates to an automated polymer synthesis apparatus for synthesizing a polymer chain onto a solid substrates by sequentially adding polymer building blocks as well as to a method for synthesizing polymers on solid substrates by sequentially reacting polymer building blocks with reactive groups. The invention further relates to a biochip comprising a solid substrate with reactive groups where biomolecules are attached to and the remaining reactive groups are transformed into chemically inert species.

Abstract: The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sampe. (I) wherein R1 is one type of oligonucleotide, Y is S or O, X is a spacer arm selected from —(CH2)—O—, —(CH2)<SB>P</SB>O—[(CH2)2—O]q—, —(CH2)r; —CO—NR—(CH2)r-0-, —CH2r—NCH3—(CH2)r—O—, —CH2)rCO—NR?—[CH2)2—O]s—, —(CH2)rNCH3[(CH2)2—O]<SB>S</SB>—, R? is H or CH3, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r? is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r? and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

Abstract: The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z?, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, ?-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associatio

Abstract: The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z?, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, ?-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

Abstract: The invention concerns composite nanospheres having a diameter ranging between about 50 and 1000 nm plus or minus 5%, preferably between about 100 and 500 nm plus or minus 5% and advantageously between 100 and 200 nm plus or minus 5%, and comprising an essentially liquid core consisting of an organic phase and inorganic nanoparticles, distributed inside the organic phase, and a skin consisting of at least a hydrophilic polymer derived from the polymerisation of at least one water soluble monomer, in particular N-alkylacrylamide or a N-N-dialkylacrylamide; conjugates derived from said nanospheres; their preparation methods and their uses.

Abstract: This invention concerns a valve (2), with at least one channel (3) running through it, allowing a fluid (F3) to be directed by transfer means within a device (1), the device (1) featuring at least one face (4), possibly flat, the valve (2) consisting of a film (7 or 16), flexible and/or which can be deformed, fixed on all or part of the face (4) of said device (1), and a film actuator (7), which enables said valve (2) to be activated or deactivated, this actuator consisting of an electrical power source. It also consists of a test sample card (1) equipped with at least one valve (2), and a process for actuating the valve (2), as described above. The invention is particularly applicable in the field of diagnosis.

Abstract: The present invention relates to a method for simultaneously detecting hybridization reactions and immunoreactions in a sample which may contain target analytes consisting of at least one nucleic acid and of at least one other ligand that is different in nature, characterized in that it comprises the steps consisting in: (i) depositing a known amount of volume of the sample diluted in a reaction buffer, onto a capture surface pre-coated with the partners for capturing said target analytes, said capture partners consisting of at least one nucleic acid probe and at least one antiligand, (ii) reacting at a temperature of between 15° C. and 60° C. and (iii) visualizing the hybridization reactions and immunoreactions thus obtained, and also to the use of this method for detecting diseases of infectious or metabolic origin or viral origin, for the industrial diagnosis of the presence of bacteria, and for identifying and/or quantifying biological molecules.

Abstract: The invention relates to the use of a protein mutation method for preparing detoxified and immunogenic mutants of the wild-type HIV-1 virus Tat-protein. The invention also relates to a method for preparing detoxified and immunogenic mutants of the wild-type HIV-1 virus Tat-protein, comprising a first step in which wild-type Tat-protein mutants are prepared, a second step in which detoxified mutants having no transcellular activity but an altered nuclear localization are screened, and a third step in which immunogenic mutants capable of inducing antibodies directed against both mutants and the wild Tat protein are screened. Steps two and three are reversible.

Abstract: The invention concerns composite nanospheres having a diameter ranging between about 50 and 1000 nm plus or minus 5%, preferably between about 100 and 500 nm plus or minus 5% and advantageously between 100 and 200 nm plus or minus 5%, and comprising an essentially liquid core consisting of an organic phase and inorganic nanoparticles, distributed inside the organic phase, and a skin consisting of at least a hydrophilic polymer derived from the polymerisation of at least one water soluble monomer, in particular N-alkylacrylamide or a N-N-dialkylacrylamide; conjugates derived from said nanospheres; their preparation methods and their uses.

Abstract: RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.

Abstract: An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.