Patents by Inventor Bernard Meijrink

Bernard Meijrink has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11149288
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Patent number: 11118193
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: September 14, 2021
    Assignee: DSM IP ASSESTS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 10865407
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: December 15, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, Herman Jan Pel, Bernard Meijrink
  • Patent number: 10793850
    Abstract: The present invention relates to a method for carrying out recombination at a target locus.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: October 6, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nathalie Wiessenhaan, Catharina Petronella Antonia Maria Kolen, Bernard Meijrink, Viktor Marius Boer, Johannes Andries Roubos, Yvonne Johannes Odilia Arendsen
  • Publication number: 20200172934
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: June 4, 2020
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20200165637
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: May 28, 2020
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS
  • Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 10590436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: March 17, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • Patent number: 9951339
    Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: April 24, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ilse De Lange, Bernard Meijrink, Johannes Andries Roubos, Siew-loon Ooi
  • Publication number: 20180023096
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 25, 2018
    Applicant: DSM IP ASSETS B.V.
    Inventors: BERNARD MEIJRINK, RENÉ VERWAAL, BIANCA ELISABETH MARIA GIELESEN, JOHANNES ANDRIES ROUBOS
  • Publication number: 20180010151
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 11, 2018
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20170314011
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Application
    Filed: July 13, 2017
    Publication date: November 2, 2017
    Inventors: Johannes Andries ROUBOS, Herman Jan Pel, Bernard Meijrink
  • Patent number: 9738890
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: August 22, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, Herman Jan Pel, Bernard Meijrink
  • Publication number: 20170191089
    Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
    Type: Application
    Filed: May 28, 2015
    Publication date: July 6, 2017
    Applicant: DSM IP Assets B.V.
    Inventors: Zheng ZHAO, Bernard MEIJRINK, Robertus Antonius Mijindert VAN DER HOEVEN, Liang WU, Johannes Andries ROUBOS
  • Publication number: 20170191091
    Abstract: The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
    Type: Application
    Filed: May 28, 2015
    Publication date: July 6, 2017
    Inventors: Zheng ZHAO, Bernard MEIJRINK, Robertus Antonius Mijndert VAN DER HOEVEN, Liang WU, Johannes Andries ROUBOS, Marina VAN GELDER
  • Publication number: 20150291986
    Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
    Type: Application
    Filed: November 25, 2013
    Publication date: October 15, 2015
    Inventors: Zheng Zhao, Bernard Meijrink, Robertus Antonius Mijndert Van Der Hoeven, Liang Wu, Johannes Andries Roubos
  • Publication number: 20150225728
    Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
    Type: Application
    Filed: September 19, 2013
    Publication date: August 13, 2015
    Inventors: Ilse De Lange, Bernard Meijrink, Johannes Andries Roubos, Siew-loon Ooi
  • Publication number: 20150050696
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 19, 2015
    Inventors: Johannes Andries Roubos, Herman Jan Pel, Bernard Meijrink
  • Publication number: 20150037892
    Abstract: The present invention relates to a method for carrying out recombination at a target locus.
    Type: Application
    Filed: March 12, 2013
    Publication date: February 5, 2015
    Inventors: Nathalie Wiessenhaan, Catharina Petronella Antonia Maria Kolen, Bernard Meijrink, Viktor Marius Boer, Johannes Andries Roubos, Yvonne Johannes Odilia Arendsen