Abstract: This invention provides a genetically manipulated cell useful for treating or preventing a malignant tumor in a patient which: (a) expresses at least one immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens; and (b) is allogeneic to the patient. This invention also provides a method of treating a malignant tumor in a subject which comprises administering to the subject a plurality of the genetically manipulated cell so as to inhibit proliferation of the malignant tumor. This invention further provides a method of preventing tumor formation in a subject comprising administering to the subject a plurality of the genetically manipulated cell so as to prevent tumor formation. Finally, this invention provides a method for making the genetically manipulated cell.

Abstract: This invention provides a retroviral vector comprising (a) a 3?LTR; and (b) an insert having (i) a promoter, (ii) a sequence encoding an immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens, and (iii) a poly A signal, respectively, said promoter, sequence encoding an immunomolecule and poly A signal being aligned in a 5? to 3? orientation, wherein the insert is positioned within the 3?LTR in the orientation opposite to that of the 3?LTR. This invention further provides a genetically manipulated cell useful for treating or preventing a malignant tumor in a patient which a) expresses at least one immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens; and b) is allogenic to the patient.

Abstract: This invention provides a genetically manipulated cell useful for treating or preventing a malignant tumor in a patient which: (a) expresses at least one immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens; and (b) is allogenic to the patient. This invention also provides a method of treating a malignant tumor in a subject which comprises administering to the subject a plurality of the genetically manipulated cell so as to inhibit proliferation of the malignant tumor. This invention further provides a method of preventing tumor formation in a subject comprising administering to the subject a plurality of the genetically manipulated cell so as to prevent tumor formation. Finally, this invention provides a method for making the genetically manipulated cell.

Abstract: This invention provides a retroviral vector comprising (a) a 3?LTR; and (b) an insert having (i) a promoter, (ii) a sequence encoding an immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens, and (iii) a poly A signal, respectively, said promoter, sequence encoding an immunomolecule and poly A signal being aligned in a 5? to 3? orientation, wherein the insert is positioned within the 3?LTR in the orientation opposite to that of the 3?LTR. This invention further provides a genetically manipulated cell useful for treating or preventing a malignant tumor in a patient which a) expresses at least one immunomolecule selected from the group consisting of cytokines, adhesion molecules, costimulatory factors, tumor associated antigens and tumor specific antigens; and b) is allogenic to the patient.