Abstract: The present technology comprises methods and RNA constructs for the targeted translation of a polypeptide of interest in a eukaryotic target cell, and to the use thereof in therapeutic clinical applications.

Abstract: A crimping frame or crimping base for a charge air cooler may include a circumferential raised rim including a plurality of crimping slots. The plurality of crimping slots may each include two longitudinal sides and two narrow sides. The two longitudinal sides may be disposed opposite one another and may extend parallel to the rim. The two narrow sides may be stiffened via a bent reinforcement. The bent reinforcement and the rim may be structured as a single piece.

Abstract: A crimping frame or crimping base for a charge air cooler may include a circumferential raised rim including a plurality of crimping slots. The plurality of crimping slots may each include two longitudinal sides and two narrow sides. The two longitudinal sides may be disposed opposite one another and may extend parallel to the rim. The two narrow sides may be stiffened via a bent reinforcement. The bent reinforcement and the rim may be structured as a single piece.

Abstract: A system for monitoring liquid intake of a user includes a monitor configured to determine an amount of liquid removed from a container, an interface configured to receive a vital parameter of the user of the system from a vital parameter sensor, and a prompter configured to prompt the user to drink as a function of the amount of liquid removed and of the vital parameter.

Abstract: The present technology comprises methods and RNA constructs for the targeted translation of a polypeptide of interest in a eukaryotic target cell, and to the use thereof in therapeutic clinical applications.

Abstract: A system for monitoring liquid intake of a user includes a monitor configured to determine an amount of liquid removed from a container, an interface configured to receive a vital parameter of the user of the system from a vital parameter sensor, and a prompter configured to prompt the user to drink as a function of the amount of liquid removed and of the vital parameter.

Abstract: A molecule mixture comprising an amphipathic molecule of type A, which has an overall positive charge in the hydrophilic region, an amphipathic molecule of type B and a polyphenol C, the method for producing the molecule mixture, and the use thereof. The molecules of types A and B are present at a molecular ratio of A:B of 1(±0.2):1(±0.2) mol/mol. A method for producing the mixture and the use is also disclosed.

Abstract: A method for producing a fusion mixture for a transfer of a charged molecule into and/or through a lipid membrane is disclosed. In an embodiment, the method comprises: providing an initial mixture comprising a positively charged amphipathic molecule A, an aromatic molecule B with hydrophobic range and a neutral, amphipathic molecule C, whereby the molecule types are at hand in a ratio A:B:C of 1-2:0.02-1:0-1 mol/mol; generating a fusogenic liposome by absorption of the initial mixture in a watery solvent; providing a charged molecule; forming a complex from the charged molecule and a neutralizing agent; and incubating the complex with the fusogenic liposome so that a fusion mixture is obtained.

Abstract: The invention relates to a fusion mixture for the lipid-containing membrane modification of an arbitrary target membrane, a cell membrane, a constituent of a cell membrane or a cell membrane separated from remaining cell constituents, in vivo or in vitro, comprising a positively charged amphipathic molecule A and an aromatic molecule B, wherein the molecule of type A and the molecule of type B are present at a ratio A:B of 1:0:02 to 1:2 mol/mol.

Abstract: A molecule mixture comprising an amphipathic molecule of type A, which has an overall positive charge in the hydrophilic region, an amphipathic molecule of type B and a polyphenol C, the method for producing the molecule mixture, and the use thereof. The molecules of types A and B are present at a molecular ratio of A:B of 1(±0.2):1(±0.2) mol/mol. A method for producing the mixture and the use is also disclosed.

Abstract: A device for examining cells comprising an elastomer and the use thereof. The elastomer comprises a bottom and a thicker edge region; and the bottom is provided with regular microstructures. Such elastomers are suitable for stretch experiments, notably of the uniaxial type.

Abstract: Disclosed is a mixture of amphipathic molecules and a method for modifying cells in vivo by way of membrane fusion with these molecules.

Abstract: A separation device has a first separation chamber for housing a packing material representing a stationary phase configured for separating compounds of a fluid sample. The separation device comprises a first separation device element and a second separation device element, configured to be coupled together to provide the first separation chamber. Each of the first separation device element and the second separation device element comprises a retaining element configured to retain the packing material within the first separation chamber. The first separation chamber is provided by the first separation device element and the second separation device, with the retaining elements of both the first separation device element and the second separation device closing the first separation chamber.

Abstract: An apparatus and method for treating a rotating printing technology surface, especially a re-imageable printing form, with a process liquid, such as cleaning, covering or rinsing liquid, include jet nozzles disposed along a straight line substantially parallel to the axis of rotation of the printing technology surface, and a cloth device substantially parallel to the axis of rotation. The nozzles pivot about a pivot axis substantially perpendicular to the axis of rotation for producing a substantially uniform distribution of process liquid parallel to the axis of rotation. The cloth device preferably has a cloth with segmented or calendered portions substantially parallel to the axis of rotation, for substantially limiting spread of process liquid in the cloth parallel to the feed direction of the cloth and assisting substantially uniform distribution of process liquid parallel to the axis of rotation. Machines for processing printing material and for treating printing forms are also provided.

Abstract: A method for treating a reusable printing technology surface, preferably a printing form that can be re-imaged, with at least one liquid, for example cleaning fluid, rinsing liquid, solution of amphiphilic molecules or gumming agent, includes rotating the printing technology surface about a cylinder axis, applying the liquid to the printing technology surface in the form of a jet and at a varying angle and contacting the printing technology surface with a movable cloth. The printing technology surface is rotated at a varying rotational speed. The rotational speed is preferably varied in stages, in particular increased, in order to achieve the best possible treatment results, for example cleaning and (re-) covering with amphiphilic molecules. The method can preferably be implemented in a printing press or a plate exposer. A machine for treating a reusable printing technology surface and a machine for processing printing material, are also provided.

Abstract: The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5?-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5? NTR was constructed and tested. Furthermore, a primer-probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity.

Abstract: The invention relates to a cell culture apparatus for cells, characterized by a surface composed of an unstructured elastomer. It is thus possible for cells to be cultured under close to natural conditions in relation to their environmental elasticity. A method for producing an apparatus according to the invention is disclosed, as is a cell culture method using such an apparatus.

Abstract: An apparatus and method for treating a rotating printing technology surface, especially a re-imageable printing form, with a process liquid, such as cleaning, covering or rinsing liquid, include jet nozzles disposed along a straight line substantially parallel to the axis of rotation of the printing technology surface, and a cloth device substantially parallel to the axis of rotation. The nozzles pivot about a pivot axis substantially perpendicular to the axis of rotation for producing a substantially uniform distribution of process liquid parallel to the axis of rotation. The cloth device preferably has a cloth with segmented or calendered portions substantially parallel to the axis of rotation, for substantially limiting spread of process liquid in the cloth parallel to the feed direction of the cloth and assisting substantially uniform distribution of process liquid parallel to the axis of rotation. Machines for processing printing material and for treating printing forms are also provided.

Abstract: The invention relates to a method for producing a structured elastomer. The invention is characterised in that the elastomer is applied and hardened directly on a structured SiO2-surface. As a result, highly-deformable, micro-structured elastomers can be produced for force sensors which are used during the characterisation of new therapeutic agents.

Abstract: A device for correcting the lateral position of a printing substrate, preferably a sheet of paper, held by a gripper bar and conveyed in a transporting direction has a linear drive that includes a first primary part and a first secondary part configured as a first carriage and associated with the first primary part, and a second secondary part configured as a second carriage. The first carriage and the second carriage are arranged on the same side of the printing substrate in the lateral direction and—to correct the position of the printing substrate in the lateral direction—are movable relative to each other in the direction of transport. Such a device may be used for transporting printing substrates and for correcting the register position of printing substrates in machines for processing printing substrates, preferably printing presses or machines for the further processing of printed products.