Abstract: The invention relates to novel cytochrome P450 monooxygenases from thermophilic bacteria, in particular the genus Thermus sp., to nucleotide sequences encoding them, to the recombinant production of these monooxygenases and to their use for the microbiological oxidation of organic compounds.

Abstract: The present invention relates to a process for the selective oxidation of toluene derivatives by microbial peroxidases from microorganisms of the genus Coprinus.

Abstract: A description is given of epoxide hydrolases from bacteria of the genus Streptomyces, a novel process for the enzymatic separation of epoxide enantiomeric mixtures, of a novel detection method for epoxide hydrolase activity, a screening method for detecting epoxide hydrolase activity and the use of bacteria of the genus Streptomyces and of the resultant epoxide hydrolases for enantioselective epoxide hydrolysis.

Abstract: This application relates to a new process for the production of R-phenylacetylcarbinol by an enzymatic process in a liquid two-phase system.

Abstract: A polypeptide with pyruvate decarboxylase activity, derived from the pyruvate decarboxylase from Zymomonas mobilis by substitution of an amino acid in position 553.

Abstract: The invention relates to a process for the biocatalytic oxidation of aromatic compounds using recombinant xylene monooxygenase-expressing microorganisms in a biphasic reaction medium.

Abstract: A process is described for analyzing enzyme-catalyzed conversions of nonpolymeric substrates to nonpolymeric products with the aid of MALDI-TOF mass spectrometry, preferably in the presence of an internal standard on a specific carrier material.

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD+ formed by reductive cleavage of oxygen is electrochemically reduced, is described.

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.

Abstract: Process for the production of R-phenylacetylcarbinol by biotransformation of benzaldehyde by filamentous fungi.

Abstract: A gene construct comprising a biotin gene having the sequence SEQ ID No. 1 or SEQ ID No. 3, organisms which comprise this gene construct, the use of these sequences or of the gene construct for preparing biotin, and a process for preparing biotin are described.

Abstract: The invention relates to a process for the oxidation of substituted trimethylcyclohexenyl compounds in the presence of microorganisms of the order Actinomycetales and to the use of microorganisms in oxidation reactions.

Abstract: The present invention relates to a novel process for the preparation, by fermentation, of compounds of the formula I ##STR1## where R.sup.1 is hydrogen, fluorine, chlorine or bromine.

Abstract: A process for hydroxylating compounds of the formula ##STR1## where n, A and R.sup.1 to R.sup.4 have the meanings stated in the description, with the aid of microorganisms of the genus Beauveria.

Abstract: A process for the biotechnological preparation of alcohols, aldehydes and carboxylic acids by oxidation of compounds of the formula R.sup.1 --C(CH.sub.3).dbd.CR.sup.2 R.sup.3, where R.sup.1, R.sup.2 and R.sup.3 have the meanings indicated in the description, with the aid of microorganisms is described.

Abstract: 2-hydroxyphenylacetic acid is prepared from phenylacetic acid by fermentation with suitable microorganisms.

Abstract: A process for producing optically active primary and secondary amines from the corresponding racemates is characterised in that (a) a racemic amine is enantioselectively acylated in the presence of a hydrolase with an ester whose acid component bears a fluorine, nitrogen, oxygen or sulphur atom at the proximity of the carbonyl carbon atom; (b) the mixture of optically active amine and optically active acylated amine is separated so that an enantiomer of amine is produced; (c) if desired the other enantiomer of the amine is extracted from the acylated amine by amide cleavage.

Abstract: A process for preparing enantiomerically pure lactams of the formula I ##STR1## where R.sup.1 and R.sup.2 are each, independently of one another, H, unsubstituted or substituted C.sub.1 -C.sub.4 -alkyl, unsubstituted or substituted C.sub.2 -C.sub.4 -alkenyl, unsubstituted or substituted aryl, (CH.sub.2).sub.n --COOH with n=0, 1, 2, 3, and X is 1, 2, 3, 4, 5, from a racemate of the formula I by. allowing a biocatalyst which selectively converts one enantiomer from I to act on the racemic mixture of I, and isolating the unconverted enantiomer from the resulting mixture of products.

Abstract: The invention relates to a novel process for preparing 3-hydroxyphenylacetic acid by cultivating a fungus of the genus Rhizoctonia, Ceratobasidium and Pellicularia in the presence of a phenyl compound of the formula I ##STR1## where X is COOR, CH.sub.2 OH, CH.sub.2 NH.sub.2 or ##STR2## R is methyl, ethyl, hydrogen, sodium, potassium or ammonium.

Abstract: A process for the oxidation of 2-phenoxypropionic acid to 2-(4-hydrophenoxy)propionic acid using microorganisms is described.