Patents by Inventor Bernhard Spensberger
Bernhard Spensberger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230391823Abstract: Herein is reported a method for the purification or production of a therapeutic polypeptide using the same depth filter multiple times, i.e. a depth filter which has been used before and has been regenerated. Reported herein is a method for purifying or producing a therapeutic polypeptide, characterized in that the method comprises the following steps: a) filtering an aqueous composition containing said therapeutic polypeptide and impurities through a depth filter, recovering the flow-through and thereby obtaining said purified therapeutic polypeptide, b) contacting said depth filter with a regeneration solution and thereby regenerating the depth filter, and c) repeating steps a) and b) one or more times.Type: ApplicationFiled: August 23, 2023Publication date: December 7, 2023Applicant: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Christoph Feistl, Thorsten Lemm, Marc Pompiati, Bernhard Spensberger
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Publication number: 20220194980Abstract: The present disclosure provides purification platforms comprising a depth filter step and/or a hydrophobic interaction chromatography (HIC) step. Also disclosed herein are methods of using the purification platforms described herein and compositions obtained therefrom, such as pharmaceutical compositions.Type: ApplicationFiled: November 2, 2021Publication date: June 23, 2022Inventors: Michael LEISS, Christian Meyer, Alexandra Thomas Morris, Bernhard Spensberger, Yinges Yigzaw, Franziska Edelmann, Roberto Falkenstein, Tobias Graf, Jie Gu, Jeevan Prabhakar
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Publication number: 20220169675Abstract: The current invention reports a method for purifying an antibody by reducing the content of a host cell protein. The method employs a wash step with a low conductivity aqueous solution in an affinity chromatography.Type: ApplicationFiled: May 24, 2021Publication date: June 2, 2022Applicants: Genentech, Inc., Hoffmann-La Roche Inc.Inventors: Paul MCDONALD, Richard ST. JOHN, Marc Wong, Roberto FALKENSTEIN, Wolfgang KOEHNLEIN, Klaus SCHWENDNER, Bernhard SPENSBERGER, Michael WIEDMANN, Frank ZETTL, Annika KLEINJANS, Carina KOPP, Benjamin TRAN, Ryan ERICKSON
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Patent number: 11332514Abstract: Herein is reported a method for purifying a polypeptide comprising the steps of i) applying a solution comprising the polypeptide to an ion exchange chromatography material, and ii) recovering the polypeptide with a solution comprising a denaturant and thereby purifying the polypeptide, whereby the ion exchange chromatography material comprises a matrix of cross-linked poly (styrene-divinylbenzene) to which ionic ligands have been attached, and wherein the solution comprising the polypeptide applied to the ion exchange chromatography material is free of the denaturant and the polypeptide adsorbed to the ion exchange chromatography material is recovered with a solution comprising a denaturant at a constant conductivity.Type: GrantFiled: October 22, 2015Date of Patent: May 17, 2022Assignee: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Maria Laura Magri, Michaela Mehr, Klaus Schwendner, Bernhard Spensberger
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Publication number: 20220119499Abstract: Herein is reported a method for producing a human IgG4 or IgG1 isotype antibody comprising the following steps a) cultivating a cell comprising a nucleic acid encoding a human IgG4 or IgG1 isotype antibody, b) recovering the human IgG4 or IgG1 isotype antibody from the cell or the cultivation medium, c) contacting the human IgG4 or IgG1 isotype antibody with a protein A chromatography material, d) washing the protein A chromatography material with an aqueous solution comprising Histidine and Tris, e) recovering the human IgG4 or IgG1 isotype antibody from the protein A chromatography material and thereby producing the human IgG4 or IgG1 isotype antibody.Type: ApplicationFiled: June 1, 2021Publication date: April 21, 2022Applicant: Hoffmann-La Roche Inc.Inventors: Roberto FALKENSTEIN, Wolfgang KOEHNLEIN, Klaus SCHWENDNER, Bernhard SPENSBERGER
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Publication number: 20210155655Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.Type: ApplicationFiled: January 11, 2021Publication date: May 27, 2021Applicant: Hoffmann-La Roche Inc.Inventors: ROBERTO FALKENSTEIN, BERNHARD SPENSBERGER
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Publication number: 20210155656Abstract: Herein is reported a method for purifying a protein from a sample comprising the protein and at least one impurity with hydrolytic activity, comprising the following steps: i) a) applying the sample comprising the protein and at least one impurity with hydrolytic activity to a chromatography material, b) applying a solution which comprises a polysorbate to the chromatography material, and c) recovering the protein from the chromatography material, or ii) a) adding a polysorbate to the sample comprising the protein and at least one impurity with hydrolytic activity, b) applying the mixture of step a) to a chromatography material, and c) recovering the protein from the chromatography material, or iii) a) adding a polysorbate to the sample comprising the protein and at least one impurity with hydrolytic activity, b) applying the mixture of step a) to a chromatography material, c) applying a solution which comprises a polysorbate to the chromatography material, and d) recovering the protein from the chrType: ApplicationFiled: October 26, 2020Publication date: May 27, 2021Applicant: Hoffmann-La Roche Inc.Inventors: Roberto FALKENSTEIN, Michael LEISS, Bernhard SPENSBERGER, Satya Krishna Kishore RAVURI
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Publication number: 20190300567Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.Type: ApplicationFiled: January 10, 2019Publication date: October 3, 2019Applicant: Hoffmann-La Roche Inc.Inventors: ROBERTO FALKENSTEIN, BERNHARD SPENSBERGER
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Patent number: 10087237Abstract: Herein is reported a method for producing an antibody preparation comprising the steps of a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain bound to the cation exchange chromatography material, and c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 10%.Type: GrantFiled: November 6, 2015Date of Patent: October 2, 2018Assignee: F. HOFFMANN-LA ROCHE AGInventors: Roberto Falkenstein, Klaus Schwendner, Bernhard Spensberger
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Publication number: 20180186865Abstract: Herein is reported a method for producing a human IgG4 or IgG1 isotype antibody comprising the following steps a) cultivating a cell comprising a nucleic acid encoding a human IgG4 or IgG1 isotype antibody, b) recovering the human IgG4 or IgG1 isotype antibody from the cell or the cultivation medium, c) contacting the human IgG4 or IgG1 isotype antibody with a protein A chromatography material, d) washing the protein A chromatography material with an aqueous solution comprising Histidine and Tris, e) recovering the human IgG4 or IgG1 isotype antibody from the protein A chromatography material and thereby producing the human IgG4 or IgG1 isotype antibody.Type: ApplicationFiled: February 20, 2018Publication date: July 5, 2018Applicant: Hoffmann-La Roche Inc.Inventors: Roberto FALKENSTEIN, Wolfgang KOEHNLEIN, Klaus SCHWENDNER, Bernhard SPENSBERGER
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Publication number: 20180186832Abstract: The current invention reports a method for purifying an antibody by reducing the content of a host cell protein. The method employs a wash step with a low conductivity aqueous solution in an affinity chromatography.Type: ApplicationFiled: February 20, 2018Publication date: July 5, 2018Applicants: Genentech, Inc., Hoffmann-La Roche IncInventors: Paul MCDONALD, Richard ST. JOHN, Marc WONG, Roberto FALKENSTEIN, Wolfgang KOEHNLEIN, Klaus SCHWENDNER, Bernhard SPENSBERGER, Michael WIEDMANN, Frank ZETTL, Annika KLEINJANS, Carina KOPP, Benjamin TRAN, Ryan ERICKSON
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Publication number: 20170066814Abstract: Herein is reported a method for producing an antibody preparation comprising the steps of a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain bound to the cation exchange chromatography material, and c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 10%.Type: ApplicationFiled: November 6, 2015Publication date: March 9, 2017Applicant: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Klaus Schwendner, Bernhard Spensberger
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Publication number: 20160237140Abstract: Herein is reported a method for purifying a polypeptide comprising the steps of i) applying a solution comprising the polypeptide to an ion exchange chromatography material, and ii) recovering the polypeptide with a solution comprising a denaturant and thereby purifying the polypeptide, whereby the ion exchange chromatography material comprises a matrix of cross-linked poly (styrene-divinylbenzene) to which ionic ligands have been attached, and wherein the solution comprising the polypeptide applied to the ion exchange chromatography material is free of the denaturant and the polypeptide adsorbed to the ion exchange chromatography material is recovered with a solution comprising a denaturant at a constant conductivity.Type: ApplicationFiled: October 22, 2015Publication date: August 18, 2016Applicant: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Maria Laura Magri, Michaela Mehr, Klaus Schwendner, Bernhard Spensberger
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Publication number: 20140162317Abstract: Herein is reported a method for producing an antibody preparation comprising the steps of a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain bound to the cation exchange chromatography material, and c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 10%.Type: ApplicationFiled: December 19, 2011Publication date: June 12, 2014Inventors: Roberto Falkenstein, Kaus Schwendner, Bernhard Spensberger
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Publication number: 20130053551Abstract: Herein is reported a method for purifying a polypeptide comprising the steps of i) applying a solution comprising the polypeptide to an ion exchange chromatography material, and ii) recovering the polypeptide with a solution comprising a denaturant and thereby purifying the polypeptide, whereby the ion exchange chromatography material comprises a matrix of cross-linked poly (styrene-divinylbenzene) to which ionic ligands have been attached, and wherein the solution comprising the polypeptide applied to the ion exchange chromatography material is free of the denaturant and the polypeptide adsorbed to the ion exchange chromatography material is recovered with a solution comprising a denaturant at a constant conductivity.Type: ApplicationFiled: August 17, 2012Publication date: February 28, 2013Applicant: Hoffman-La Roche. Inc.Inventors: Roberto Falkenstein, Maria Laura Magri, Michaela Mehr, Klaus Schwendner, Bernhard Spensberger