Abstract: An approach to collect and interpret results from Y2H screens uses high-throughput next-generation sequencing technologies. In particular, this system is appropriate to generate comprehensive profiles of protein-protein interactions (PPIs), allowing also a side-by-side comparison of specific PPI patterns with that from control samples and allows a direct comparison of PPI patterns displayed by proteins in their wild-type and various mutant conformations. While sample preparation relies to the most part on established transcriptome and RNA sequencing procedures, this invention also encloses a specific DNA preparation step to sequester irrelevant and contaminating sequences from the sample.

Abstract: An approach to collect and interpret results from Y2H screens uses high-throughput next-generation sequencing technologies. In particular, this system is appropriate to generate comprehensive profiles of protein-protein interactions (PPIs), allowing also a side-by-side comparison of specific PPI patterns with that from control samples and allows a direct comparison of PPI patterns displayed by proteins in their wild-type and various mutant conformations. While sample preparation relies to the most part on established transcriptome and RNA sequencing procedures, this invention also encloses a specific DNA preparation step to sequester irrelevant and contaminating sequences from the sample.

Abstract: A method for a client to locate a particular service from a service provider on wide area computer networks. The method includes multicasting of an advertisement from a service provider, which advertisement is detected by a Service Broker and in turn multicast into the wide area computer network. A client queries the network when seeking a particular service and receives in turn the address of the Broker and a Server to obtain the service desired.

Abstract: A per-flow queuing method and apparatus for IP networks carrying traffic from feedback controlled TCP connections enables flow of information packets from one or more sources to a destination through a link and comprises a buffer of predetermined size partitioned into a plurality of queues, each queue being allocated an occupancy b.sub.i for receiving and temporarily storing packets of information; a scheduler for removing packets from each buffer according to a predetermined rate and transmitting the packets over a network; and a control device for determining availablilty of queues in the buffer capable of receiving the packet and inputting the packet into a queue if the queue is available, the control device further selecting a queue and releasing a packet from the selected queue to accommodate input of the received packet when the queue is not available.

Abstract: A data transmission control arrangement for an asymmetric communication system having a fast downstream link enabling communication of information packets from a service provider to one or more subscriber terminal connected through a common network terminal, and a slow upstream link including transmitting packets from a subscriber terminal through the network terminal to the service provider, the data transmission control arrangement including a classifier device for classifying information packets received by the network terminal for transmission over the upstream link, the information including data packets and acknowledgement packets; a control device for establishing one or more upstream link connection queues in the network terminal in accordance with the classification of the information packet to be transmitted, the upstream connection queues including one or more data queues for transmitting data information and ACK queues for transmitting ACK packets in response to data packets communicated over the