Patents by Inventor Beth Lanning

Beth Lanning has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for measuring cysteine and determining cysteine synthase activity
    Patent number: 6605459
    Abstract: Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine but not to O-acetyl serine or sulfide; exciting the conjugate with UV light; and detecting fluorescent light emitted by the conjugate. Cysteine synthase activity is determined by combining O-acetyl-L-serine, sulfide and cysteine synthase to form a reaction mixture under conditions suitable for cysteine production; contacting the reaction mixture with an appropriate coumarin dye; subjecting the reaction mixture to UV light; and detecting fluorescent light emission.
    Type: Grant
    Filed: July 13, 2001
    Date of Patent: August 12, 2003
    Assignee: Paradigm Genetics, Inc.
    Inventors: John Rice, Beth Lanning, John Crawford, Gordon Nye
  • Methods for measuring cysteine and determining cysteine synthase activity
    Publication number: 20030087327
    Abstract: Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine but not to O-acetyl serine or sulfide; exciting the conjugate with UV light; and detecting fluorescent light emitted by the conjugate. Cysteine synthase activity is determined by combining O-acetyl-L-serine, sulfide and cysteine synthase to form a reaction mixture under conditions suitable for cysteine production; contacting the reaction mixture with an appropriate coumarin dye; subjecting the reaction mixture to UV light; and detecting fluorescent light emission.
    Type: Application
    Filed: July 13, 2001
    Publication date: May 8, 2003
    Inventors: John Rice, Beth Lanning, John Crawford, Gordon Nye
  • Methods and compositions for the identification of modulators of deoxyxylulose 5-phosphate synthase activity
    Publication number: 20020168743
    Abstract: The invention is directed to methods and compositions for the determination of deoxyxylulose 5-phosphate synthase (DXPS) activity. The methods and compositions of the invention are amenable to high throughput screening assays for the identification of inhibitors and enhancers of DXPS activity. Such compounds have use in the modulation of plant and microbial growth. The compositions of the invention are DXPS fragments and chimeric polypeptides that have increased solubility as compared to the wild type DXPS polypeptide. These DXPS fragments and chimeric polypeptides, or variants thereof, can be recombinantly expressed and purified in quantities suitable for high throughput screening assays. The assays of the invention are based on the detection of substrates of DXPS that remain after a DXPS reaction.
    Type: Application
    Filed: October 26, 2001
    Publication date: November 14, 2002
    Inventors: John W. Rice, Andreas S. Kloti, John M. Crawford, Beth Lanning, Sandy J. Stewart
  • Methods for determining deoxyxylulose 5-phosphate synthase activity
    Patent number: 6326164
    Abstract: The invention is directed to methods and compositions for the determination of deoxyxylulose 5-phosphate synthase (DXPS) activity. The methods and compositions of the invention are amenable to high-throughput screening assays for the identification of inhibitors and enhancers of DXPS activity. Such compounds have use in the modulation of plant and microbial growth. The compositions of the invention are DXPS fragments and chimeric polypeptides that have increased solubility as compared to the wild-type DXPS polypeptide. These DXPS fragments and chimeric polypeptides can be recombinantly expressed and purified in quantities suitable for high-throughput screening assays. The assays of the invention are based on the detection of substrates of DXPS that remain after a DXPS reaction.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: December 4, 2001
    Assignee: Paradigm Genetics, Inc.
    Inventors: John W. Rice, Andreas S. Kloti, John M. Crawford, Jr., Beth Lanning, Sandy J. Stewart